Volume 8, Number 12—December 2002
Research
Efficacy of Killed Virus Vaccine, Live Attenuated Chimeric Virus Vaccine, and Passive Immunization for Prevention of West Nile virus Encephalitis in Hamster Model
Robert B. Tesh*
, Juan Arroyo†, Amelia P.A. Travassos da Rosa*, Hilda Guzman*, Shu-Yuan Xiao*, and Thomas P. Monath†
, Juan Arroyo†, Amelia P.A. Travassos da Rosa*, Hilda Guzman*, Shu-Yuan Xiao*, and Thomas P. Monath†
Table 4
Antibody response of adult golden hamsters to intramuscular inoculation of 1.8 X 106 of ChimeriVax–WNV and subsequent challenge with WNV (104.0 TCID50 given intraperitoneally)a
| Animal no. |
HI antibody titer |
CF antibody titer |
WNV PRN
antibody
titerb |
||
|---|---|---|---|---|---|
| YFV
antigen |
WNV
antigen |
YFV
antigen |
WNV
antigen |
||
| 31 days after ChimeriVax-WNV | |||||
| H-8183 | 1:40 | 1:160 | 0c | 0 | 1:160 |
| H-8184 | 1:40 | 1:160 | 0 | ND | 1:160 |
| H-8185 | 1:40 | 1:160 | 0 | 1:20 | 1:640 |
| H-8186 | 1:80 | 1:320 | 0 | 1:40 | 1:320 |
| H-8187 | 1:40 | 1:160 | 0 | 1:20 | >1:640 |
| H-8188 | 1:40 | 1:160 | 0 | 1:20 | 1:640 |
| H-8189 | 1:80 | 1:320 | 0 | 1:40 | 1:640 |
| H-8190 | 1:40 | 1:160 | 0 | 1:20 | 1:320 |
| H-8191 | 1:80 | 1:160 | 0 | 1:20 | 1:80 |
| H-8192 | 1:40 | 1:160 | 0 | 1:40 | 1:80 |
| 6 days after challenge with WNV | |||||
| H-8183 | 1:20 | 1:160 | 0 | 0 | ND |
| H-8184 | 1:40 | 1:160 | 0 | 1:40 | ND |
| H-8185 | 1:20 | 1:160 | 0 | 1:20 | ND |
| H-8186 | 1:80 | 1:320 | 0 | 1:40 | ND |
| H-8187 | 1:20 | 1:160 | 0 | 1:20 | ND |
| H-8188 | 1:40 | 1:160 | 0 | 1:20 | ND |
| H-8189 | 1:80 | 1:320 | 0 | 1:40 | ND |
| H-8190 | 1:40 | 1:320 | 0 | 1:40 | ND |
| H-8191 | 1:40 | 1:320 | 0 | 1:20 | ND |
| H-8192 | 1:40 | 1:160 | 0 | 1:20 | ND |
aWNV, West Nile virus; HI, hemagglutination inhibition; CF, complement fixation; PRN, plaque reduction neutralization; YFV, Yellow fever virus; ND, not done.
bHighest serum dilution producing >90% plaque inhibition.
c0 = <1:20.
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