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Volume 8, Number 9—September 2002
Dispatch

Detection by Enzyme-Linked Immunosorbent Assay of Antibodies to West Nile virus in Birds

Gregory D. Ebel*Comments to Author , Alan P. Dupuis*, David Nicholas*, Donna Young*, Joseph Maffei*, and Laura D. Kramer*
Author affiliations: *Wadsworth Center, New York State Department of Health, Slingerlands, New York, USA;

Main Article

Table

Comparison of serologic assay results with reactivity across a range of avian orders and species

Common Name Species Order Indirect ELISA P/Na PRNT titer
House Sparrow # 1 Passer domesticus Passeriformes 3.0 40
House Sparrow # 2 P. domesticus Passeriformes 3.0 20
House Sparrow # 3 P. domesticus Passeriformes 7.2 80
Northern Mockingbird Mimus polyglottos Passeriformes 2.1 >10
European Starling Sturnus vulgaris Passeriformes 3.0 40
American Crow #1 Corvus brachyrhynchos Passeriformes 2.8 80
American Crow #2 C. brachyrhynchos Passeriformes 2.0 20
Rock Dove #1 Columba livia Columbiformes 2.9 80
Rock Dove #2 C. livia Columbiformes 2.5 320
Rock Dove #3 C. livia Columbiformes 7.6 10
White-naped Crane Grus vipio Gruiformes 5.3 >640
Waldrapp Ibis #1 Gerontica eremita Ciconiiformes 9.4 320
Waldrapp Ibis #2 G. eremita Ciconiiformes 11.4 640
Waldrapp Ibis #3 G. eremita Ciconiiformes 4.0 80
Black Crowned Night Heron Nycticorax nycticorax Ciconiiformes 5.2 160
Flamingo #1 Phoenicopterus chilensis Phoenicopteriformes 3.7 160
Flamingo #2 P. chilensis Phoenicopteriformes 4.2 >640
Malay Great Argus Argusianus argus Galliformes 7.3 40
Kenya Crested Guineafowl Guttera edouardi Galliformes 6.7 80
Wild Turkey Meleagris gallopavo Galliformes 6.0 320
Bulwer’s Pheasant Lophura bulweri Galliformes 3.5 >640
American White Pelican Pelecanus erythrorhynchos Pelecaniformes 5.7 >640
Guanay Cormorant Phalacrocorax bougainvillii Pelecaniformes 2.1 80
Brown Pelican Pelecanus occidentalis Pelecaniformes 3.8 160
Domestic Goose Anser sp. Anseriformes 3.6 40
Trumpeter Swan Cygnus buccinator Anseriformes 4.0 160
Barred Owl Strix varia Strigiformes 2.6 160
Ostrich Struthio camelus Struthioniformes 3.9 640
Magellanic Penguin Spheniscus magellanicus Sphenisciformes 4.3 >640
Black-Necked Crane Grus nigricollis Gruiformes 2.4 80
Laughing Gull
Larus atricilla
Charadriiformes
6.9
320
Domestic Duck Anas sp. Anseriformes 1.1 <10
Canada Goose Branta canadensis Anseriformes 1.2 <20
Domestic chicken Gallus gallus Galliformes 0.9 <10
SLEV-positive chicken G. gallus Galliformes 2.5 20
WEEV-positive chicken G. gallus Galliformes 1.3 <20

WNV-negative specimens shown below bold line
aELISA, enzyme-linked immunosorbent assay; P/N, positive/negative ratio; PRNT, plaque-reduction neutralization tests; SLEV, St. Louis encephalitis virus; WEEV, Western equine encephalomyelitis virus.

Main Article

Page created: July 16, 2010
Page updated: July 16, 2010
Page reviewed: July 16, 2010
The conclusions, findings, and opinions expressed by authors contributing to this journal do not necessarily reflect the official position of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.
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