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Volume 9, Number 11—November 2003
Dispatch

Fluoroquinolone Susceptibility of Campylobacter Strains, Senegal

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Eric Cardinale*Comments to Author , Jacques-Albert Dromigny†, Fatou Tall*, Maguatte Ndiaye*, Mamady Konte*, and Jean David Perrier-Gros-Claude†
Author affiliations: *ISRA-LNERV, Dakar, Sénégal; †Institut Pasteur, Dakar, Sénégal

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Abstract

To assess fluoroquinolone susceptibility of Campylobacter strains in Senegal, skin samples were collected from 250 chicken carcasses from January 2001 to October 2002. Among 205 isolated Campylobacter strains, 59% and 41% were identified as Campylobacter jejuni and C. coli, respectively; the overall ciprofloxacin-resistance rate was 34%.

Campylobacter species are one of the most common causes of human bacterial diarrhea in industrialized and developing countries (13). Campylobacter jejuni and C. coli are the two main species involved in human infections (1,2). Because of genotyping and serotyping analysis, several studies have confirmed that poultry can be a source of Campylobacter infection in humans (1,4). The contamination may be due to direct ingestion of undercooked food or cross-contamination of raw poultry to other foods by nonhygienic handling (e.g., unwashed hands, dirty utensils) (1).

No antimicrobial drug therapy is usually required for Campylobacter infections, since they are of short duration, clinically mild, and self-limiting. However, antimicrobial drug treatment is indicated for severe infections or persons at risk such as children or immunocompromised patients (1,2), especially in Africa where AIDS has reached epidemic proportions.

Fluoroquinolones are effective synthetic antimicrobial drugs used for treatment of a wide variety of bacterial infections, including campylobacteriosis (5). Because of heavy use of fluoroquinolones in veterinary medicine during the 1990s, numerous fluoroquinolone-resistant Campylobacter strains are emerging both in animals and in humans (1). We report the first evidence of ciprofloxacin-resistant Campylobacter strains isolated from chickens in Senegal, West Africa.

The Study

From January 2001 to October 2002, a total of 250 chicken carcasses were collected in Dakar, the capital of Senegal, and its suburbs. Chicken carcasses originated from broiler chickens from semi-industrialized areas. These chickens had been raised domestically; no imported animal was included in the study. Chicken carcasses were selected from a random sampling of 80 retail shops and slaughterhouses. In Senegal, the slaughtering process is manual, rudimentary, and often performed by a single person outside in poor hygienic conditions. Each carcass was examined for Campylobacter.

Standard methods for isolating Campylobacter from the skin were used, and species identification was performed by polymerase chain reaction (4). One colony per positive culture was randomly selected. The strains were maintained as stock culture in glycerol at –80°C and tested further by the E test method for susceptibility to ciprofloxacin (6). As previously reported for Campylobacter, the breakpoint used for resistance was >4 μg/mL for ciprofloxacin (7). The chi-square test was used for statistical analysis (EpiInfo, version 6, Centers for Disease Control and Prevention, Atlanta, GA). A p value of <0.05 was considered statistically significant.

Campylobacter were isolated from 82% of the samples: 120 C. jejuni and 85 C. coli were identified. MIC performed by E test showed that 34% of Campylobacter strains were resistant to ciprofloxacin without a significant difference between the two species (Table): 71% and 79% of C. coli– and C. jejuni–resistant strains, respectively, displayed a ciprofloxacin MIC >32 μg/mL. These rates were also statistically similar and showed a high level of resistance to ciprofloxacin in strains isolated in Dakar (Table).

Our data showed a significant isolation rate of Campylobacter strains (82%). In every retail shop and slaughterhouse, at least one chicken in the three or four randomly selected harbored a Campylobacter strain. As previously reported (8), C. jejuni was more frequently isolated than C. coli (59% vs. 41%). During the microbiologic analysis, one colony per culture was selected and identified. Since different strains and even species of Campylobacter can frequently be isolated from poultry products (7), the number of species isolated in our survey might be underestimated.

Conclusions

High levels of Campylobacter isolation from chicken carcasses and retail chickens have been previously reported in industrialized countries: 46% in Germany (9), 46% in Japan (10), and 73% to 100% in the United States (11). Although little information is available from developing countries, our results are consistent with those from Kenya, where thermophilic Campylobacter organisms were isolated from 77% of chicken samples (12). These results showed that the Campylobacter prevalence in chicken carcasses in some African countries may be similar to those observed in industrialized countries.

A high frequency of ciprofloxacin-resistant Campylobacter strains was observed in our survey. The prevalence of resistant strains was similar for Campylobacter isolated from chickens in Lebanon (13), the United States (11), and Japan (14). The prevalence was lower than the rate of resistant strains from Belgium (7) or Spain (2), where 100% of C. coli and 98.7% of C. jejuni, respectively, were fluoroquinolone resistant. In Taiwan, Thailand, and Spain, fluoroquinolone-resistance rates, ranging from 56% to 84%, were similar in poultry meat and human isolates (1,2). By contrast, in some countries such as Chile, no ciprofloxacin-resistant Campylobacter strains have been described (6).

Since 1991, when Endzt and colleagues (15) identified the first quinolone-resistant Campylobacter strains in C. jejuni and C. coli in the Netherlands, Campylobacter resistance to fluoroquinolones has increased throughout the world (2,6,8). This important development in humans and other animals is related to the introduction of fluoroquinolones in veterinary medicine (15). In Senegal, fluoroquinolones (enrofloxacin, norfloxacin) were introduced in veterinary medicine in 1996 in poultry production to treat respiratory and intestinal diseases. Because of the absence of any antibiotic resistance studies in Senegal, correlating the currently observed ciprofloxacin resistance with the introduction of ciprofloxacin into the country is difficult. However, our results show, as in industrialized countries, that fluoroquinolone resistance may become a public health problem for developing countries. Further studies are needed to evaluate how commonly fluoroquinolone resistance occurs among human isolates of Campylobacter in Senegal, and to what extent poultry contributes.

Because of their wide treatment spectrum, which includes bacterial infections such as salmonellosis and shigellosis, fluoroquinolones have progressively become the empiric treatment for human gastroenteritis in Senegal. In the region of Dakar, fluoroquinolones are used in all facets of poultry production, including for chickens used for broiling, laying, and breeding. Fluoroquinolones also tend to be the most prescribed drug for treatment of respiratory and intestinal diseases among poultry (approximately 25% of the antimicrobial drugs delivered in veterinary use; C. Biagui, unpub. data). To decrease ciprofloxacin-resistant Campylobacter and preserve the effectiveness of fluoroquinolones, their use in veterinary medicine should be more strictly controlled. Our finding of fluoroquinolone-resistant Campylobacter in chickens suggests the epidemiology of fluoroquinolone-resistant Campylobacter in Senegal is the same as in other countries.

Dr. Cardinale has been chief of the Poultry Pathology Laboratory at the Institut Sénégalais de Recherches Agricoles–Laboratoire national d’Elevage et de Recherches vétérinaires in Senegal since 1997. His primary scientific interests are food safety and food hygiene, epidemiology, and microbiology.

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Acknowledgments

We are grateful to members of the Association of Poultry Technicians for their help in the field.

This work was supported by the French Embassy in Dakar, Senegal.

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References

  1. Engberg  J, Aarestrup  FM, Taylor  DE, Gerner-Smidt  P, Nachamkin  I. Quinolone and macrolide resistance in Campylobacter jejuni and C. coli: resistance mechanisms and trends in human isolates. Emerg Infect Dis. 2001;7:2434. DOIPubMed
  2. Saenz  Y, Zarazaga  M, Lantero  M, Gastanares  MJ, Baquero  F, Torres  C. Antibiotic resistance in Campylobacter strains isolated from animals, foods, and humans in Spain in 1997–1998. Antimicrob Agents Chemother. 2000;44:26771. DOIPubMed
  3. Smith  SI, Sansa  TI, Coker  AO. Antibiotic susceptibility patterns and beta-lactamase production of animal and human isolates of Campylobacter in Lagos, Nigeria. Z Naturforsch. 1999;54:5836.PubMed
  4. Refregier-Petton  J, Rose  N, Denis  M, Salvat  G. Risk factors for Campylobacter spp contamination in French broiler-chicken flocks at the end of the rearing period. Prev Vet Med. 2001;50:89100. DOIPubMed
  5. DuPont  HL, Ericsson  CD, Robinson  A, Johnson  PC. Current problems in antimicrobial therapy for bacterial enteric infection. Am J Med. 1987;82(4A):3248.PubMed
  6. Fernandez  H, Mansilla  M, Gonzalez  V. Antimicrobial susceptibility of Campylobacter jejuni subsp. jejuni assessed by E-test and double dilution agar method in Southern Chile. Mem Inst Oswaldo Cruz. 2000;95:2479. DOIPubMed
  7. Van Looveren  M, Daube  G, De Zutter  L, Dumont  JM, Lammens  C, Wijdooghe  M, Antimicrobial susceptibilities of Campylobacter strains isolated from food animals in Belgium. J Antimicrob Chemother. 2001;48:23540. DOIPubMed
  8. Aarestrup  FM, Bager  F, Jensen  NE, Madsen  M, Meyling  A, Wegener  HC. Resistance to antimicrobial agents used for animal therapy in pathogenic-, zoonotic- and indicator bacteria isolated from different food animals in Denmark: a baseline study for the Danish Integrated Antimicrobial Resistance Monitoring Programme (DANMAP). APMIS. 1998;106:74570. DOIPubMed
  9. Atanassova  V, Ring  C. Prevalence of Campylobacter spp. in poultry and poultry meat in Germany. Int J Food Microbiol. 1999;51:18790. DOIPubMed
  10. Ono  K, Yamamoto  K. Contamination of meat with Campylobacter jejuni in Saitama, Japan. Int J Food Microbiol. 1999;47:2119. DOIPubMed
  11. White  PL, Baker  AR, James  WO. Strategies to control Salmonella and Campylobacter in raw poultry products. Rev Sci Tech. 1997;16:52541.PubMed
  12. Osano  O, Arimi  SM. Retail poultry and beef as sources of Campylobacter jejuni. East Afr Med J. 1999;76:1413.PubMed
  13. Talhouk  RS, el-Dana  RA, Araj  GF, Barbour  E, Hashwa  F. Prevalence, antimicrobial susceptibility and molecular characterization of Campylobacter isolates recovered from humans and poultry in Lebanon. J Med Liban. 1998;46:3106.PubMed
  14. Chuma  T, Ikeda  T, Maeda  T, Niwa  H, Okamoto  K. Antimicrobial susceptibilities of Campylobacter strains isolated from broilers in the southern part of Japan from 1995 to 1999. J Vet Med Sci. 2001;63:10279. DOIPubMed
  15. Endtz  HP, Mouton  RP, van der Reyden  T, Ruijs  GJ, Biever  M, van Klingeren  B. Fluoroquinolone resistance in Campylobacter spp isolated from human stools and poultry products. Lancet. 1990;335:787. DOIPubMed

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DOI: 10.3201/eid0911.020693

Table of Contents – Volume 9, Number 11—November 2003

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Eric Cardinale, CIRAD-EMVT, ISRA-LNERV BP 2057 Dakar-Hann, Sénégal; fax: (221) 821 18 79

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