Volume 9, Number 3—March 2003
Research
Molecular Detection of Bartonella quintana, B. koehlerae, B. henselae, B. clarridgeiae, Rickettsia felis, and Wolbachia pipientis in Cat Fleas, France
Jean-Marc Rolain*, Michel Franc†, Bernard Davoust‡, and Didier Raoult*
Table 1
Oligonucleotide primers used for polymerase chain reaction amplification and sequencing
| Primer (reference) | Nucleotide sequence | Detected organism | References |
|---|---|---|---|
| CS-877 (gltA gene) | GGG GGC CTG CTC ACG GCG G | Rickettsia species | (17) |
| CS-1273 (gltA gene) BM59 (rOmpB gene) B807 (rOmpB gene) | ATT GCA AAA AGT ACA GTG AAC A CCG CAG GGT TGG TAA CTG C CCT TTT AGA TTA CCG CCT AA | Rickettsia species Rickettsia species Rickettsia species | (17) (18) (18) |
| URBarto1 (its gene) | CTT CGT TTC TCT TTC TTC A | Bartonella species | (19) |
| URBarto2 (its gene) | CTT CTC TTC ACA ATT TCA AT | Bartonella species | (19) |
| PAPn1 (pap31 gene) | TTC TAG GAG TTG AAA CCG AT | Bartonella species | (20) |
| PAPn2 (pap31 gene) | GAA ACA CCA CCA GCA ACA TA | Bartonella species | (20) |
| BartogltAForward (gltA gene) | TTC CGY CTT ATG GGT TTT GG | Bartonella species | This report |
| Bartokoehlerae (gltA gene) | AAC AAA ATA TTC ATC ATT CAG G | B. koehlerae | This report |
| Bartoclarridgeiae (gltA gene) | AAA GCA ATT TTT TCA AGT TCC | B. clarridgeiae | This report |
| Bartohenselae (gltA gene) | CAT TTC TGT TGG AAA TCC TAG | B. henselae | This report |
| Bartoquintana (gltA gene) EHR16SD (16S rRNA gene) EHR16SD (16S rRNA gene) | TTT TAA TGT AAT GCC AGA ATA A GGT ACC YAC AGA AGA AGT CC TAG CAC TCA TCG TTT ACA GC | B. quintana Wolbachia Wolbachia | This report (21) (21) |
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