Volume 10, Number 12—December 2004
Research
Nonsusceptibility of Primate Cells to Taura Syndrome Virus
Carlos R. Pantoja*
, Solangel A. Navarro*, Jaime Naranjo*, Donald V. Lightner*, and Charles P. Gerba*
, Solangel A. Navarro*, Jaime Naranjo*, Donald V. Lightner*, and Charles P. Gerba*
Table
Mean TSV quantification in tissue cell culture mediaa,b
| Cell type | Source of inoculum | Inoculum type | Mean viral quantification (viral copies/μL) postexposure |
||
|---|---|---|---|---|---|
| Day 0 | Day 4 | Day 7 | |||
| BGMK | Hemolymph | TSV-infected | 2.7 x 106 | 2.4 x 105 | 2.5 x 104 |
| SPFc | 0 | 0 | 0 | ||
| Hepatopancreas | TSV-infected | 1.2 x 104 | 5.0 x 103 | 5.0 x 103 | |
| SPF | 0 | 0 | 0 | ||
| FrhK-4 | Hemolymph | TSV-infected | 1.3 x 105 | 1.9 x 104 | 4.7 x 103 |
| SPF | 0 | 0 | 0 | ||
| Hepatopancreas | TSV-infected | 1.3 x 104 | 8.4 x 103 | 3.7 x 103 | |
| SPF | 0 | 0 | 0 | ||
| MA-104 | Hemolymph | TSV-infected | 1.5 x 105 | 3.1 x 104 | 2.3 x 103 |
| SPF | 0 | 0 | 0 | ||
| Hepatopancreas | TSV-infected | 1.2 x 104 | 1.3 x 104 | 9.2 x 103 | |
| SPF | 0 | 0 | 0 | ||
aEstimated by real time reverse transcription–polymerase chain reaction at different intervals postexposure.
bBGMK, Buffalo green monkey kidney; FrhK-4, monkey Rhesus female kidney embryonic; MA-104, monkey African green kidney; TSV; Taura syndrome virus; SPF, specific pathogen free.
cInoculum originated from SPF penaeid shrimp.
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