Volume 10, Number 12—December 2004
Dispatch
Antibodies to SARS-Coronavirus in Civets
, and Lin-Fa Wang†
Table
Summary of serologic analyses of civet serum samplesa
| Sample no. | Farm |
Market |
||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Hunan |
Henan |
Guangdong |
Guangdong |
|||||||||||||
| Changsha |
Luoning |
Qianguan |
Shaoguan |
Shanwei |
Zhuhai |
Guangzhou |
||||||||||
| VNTb | IFAc | VNTb | IFAc | VNTb | IFAc | VNTb | IFAc | VNTb | IFAc | WBd | VNTb | IFAc | VNTb | IFAc | WBd | |
| 1 | – | – | – | – | – | – | NA | NA | 10 | – | – | – | – | 10 | +++ | ++++ |
| 2 | – | – | – | – | – | – | – | – | 10 | + | + | – | – | 640 | ++ | ++++ |
| 3 | – | – | – | – | NA | NA | – | – | – | – | – | – | – | CONT | +++ | +++ |
| 4 | – | – | – | – | – | – | – | – | – | – | – | – | – | 20 | +++ | ++++ |
| 5 | – | – | – | – | – | – | – | – | 40 | +/– | + | – | – | – | – | – |
| 6 | – | – | – | – | – | – | – | – | 20 | – | – | – | – | 30 | ++ | +++ |
| 7 | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| 8 | – | – | – | – | – | – | – | – | – | – | – | – | – | 10 | ++ | +++ |
| 9 | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | + |
| 10 | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| 11 | – | – | – | – | 10 | ++ | ++++ | |||||||||
| 12 | – | – | – | – | NA | NA | NA | |||||||||
| 13 | – | – | – | – | 20 | ++ | ++++ | |||||||||
| 14 | – | – | – | – | 30 | ++ | ++++ | |||||||||
| 15 | – | – | – | – | NA | NA | NA | |||||||||
| 16 | – | – | – | – | 10 | ++ | ++++ | |||||||||
| 17 | – | – | – | – | 10 | +++ | ++++ | |||||||||
| 18 | – | – | 10 | ++ | ++++ | |||||||||||
| 19 | – | – | 240 | +++ | ++++ | |||||||||||
| 20 | – | – | 60 | +++ | ++++ | |||||||||||
| 21 | – | – | ||||||||||||||
| 22 | – | – | ||||||||||||||
| 23 | – | – | ||||||||||||||
| 24 | – | – | ||||||||||||||
| 25 | – | – | ||||||||||||||
| 26 | – | – | ||||||||||||||
| 27 | – | – | ||||||||||||||
| 28 | – | – | ||||||||||||||
| 29 | – | – | ||||||||||||||
| 30 | – | – | ||||||||||||||
a VNT, virus neutralization test; IFA, immunofluorescent antibody test; WB, Western blot test; NA, not assayed because of sample damage or loss during transportation or storage; CONT, contaminated samples that exhibited toxicity in VNT test.
bTiter of antibody that neutralized infectivity of 200 50% tissue culture infective dose of SARS-CoV.
cImmunofluorescent antibody test was performed by using a serum dilution of 1:50, and the intensity of staining in SARS-CoV–infected cells, but not in mock infected cells, was indicated by the sign +, with +++ representing the strongest signal observed.
dWestern blot was conducted by using a serum dilution of 1:50, and the reactivity with the recombinant N protein expressed was indicated by the sign +, with ++++ representing the strongest signal observed.