Volume 10, Number 7—July 2004
Dispatch
Emergence of Multidrug-resistant Salmonella Paratyphi B dT+, Canada
Michael R. Mulvey*
, David Boyd*, Axel Cloeckaert†, Rafiq Ahmed*, Lai-King Ng, and the Provincial Public Health Laboratories1
, David Boyd*, Axel Cloeckaert†, Rafiq Ahmed*, Lai-King Ng, and the Provincial Public Health Laboratories1
Table
PCR primer to detect various regions of SGI1
| Set | Primer | Primer sequence (5′ to 3′) | Coordinatesa | Product size (bp) |
|---|---|---|---|---|
| St1 |
U9-L1
P1-R1 |
TACTACAAGCAGATAACGCC
TAGAAACGACAAAGCGCGTG |
2771–2790
3660–3679 |
909 |
| St3 |
P134-L1
P134-R2 |
AATCGACACGCGCTGTATTG
CTTCCCATAATGCCGCAATG |
16350–16369
17287–17306 |
957 |
| St4 |
P134-L1
P134-R1 |
TGACCCAATTCCAAAGCCAC
GTGTTTGGGCAAGATCCCAG |
16784–16803
17820–17839 |
1490 |
| St5 |
St2-GP21
St2-GP6 |
ATAACGGCAGGTTCCGGTTC
CGATGAAGCGCACAAATTTG |
20173–20192
21089–21108 |
936 |
| St6 | St2-GP24 St2-GP28 | TCAAGATTCCTATCTGCAGG AGAGTTACTAGACCAAGCGC | 24363–24382 25182–25201 | 838 |
aCoordinates from accession no. AF261825.
1Provincial Public Health Laboratory Members: Lewis Abbott (Prince Edward Island), Kevin Forward (Nova Scotia), Glenna Hardy (New Brunswick), Greg Horsman (Saskatchewan), Judy Isaac-Renton (British Columbia), Frances Jamieson (Ontario), Jean Joly (Quebec), Jutta K. Preiksaitis (Alberta), Sam Ratnam (Newfoundland), Paul Van Caeseele (Manitoba).
Page created: January 27, 2011
Page updated: January 27, 2011
Page reviewed: January 27, 2011
The conclusions, findings, and opinions expressed by authors contributing to this journal do not necessarily reflect the official position of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.