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Volume 10, Number 8—August 2004
Research

Serotype III Streptococcus agalactiae from Bovine Milk and Human Neonatal Infections1

John F. Bohnsack*Comments to Author , April A. Whiting*, Gabriela Martinez†, Nicola Jones‡, Elisabeth E. Adderson§, Shauna Detrick*, Anne J. Blaschke-Bonkowsky*, Naiel Bisharat‡, and Marcelo Gottschalk†
Author affiliations: *University of Utah Health Sciences Center, Salt Lake City, Utah, USA; †Faculté de Médicine Vétérinaire, Université de Montréal, Saint-Hyacinthe, Québec, Canada; ‡John Radcliffe Hospital, Oxford, United Kingdom; §St. Jude Children’s Research Hospital, Memphis, Tennessee, USA

Main Article

Table 5

Inserted sequences and sequence types of infB H allele strainsa
Size of PCR poroduct from inserted sequence sites (bp)
RAPD Isolate AW-10 hylB scpb-lmb ST
II
 RF-96-2834
 3,000b
 700c
 650d
 61
ASS-97-0701
 3,000
 700
 650
 61
AL-96-1653
 1,700e
 700
 650
 61
SF-96-6312
 3,400f
 700
 650
 61g
SF-96-4396
 1,700
 700
 650
 91
NI-96-2521
 3,000
 700
 650
 105
IV-B
 ASS-96-659
 3,000
 700
 No product
 61g
SH-96-5461
 1,700
 700
 No product
 61
Ungrouped
 AL-96-2049
 3,000
 700
 650
 61
NI-96-3329 3,000 700 650 61

aRAPD, randomly amplified polymorphic DNA; ST, sequence type.
bA 3,000-bp amplicon was sequenced and found to contain an IS3-like insertion sequence interrupting GBSi1.
cA 700-bp product from the hylB site indicates that there is no inserted sequence in the site.
dA 650-bp product from the scpb-lmb site indicates that there is no inserted sequence in the site.
eA 1,700-bp product was amplified and found to contain an intact copy of GBSi1.
fA 3,400-bp amplicon was sequenced and found to contain GBSi1 and an IS1563-like insertion sequence.
gThe glcK (glucose kinase) gene in these two strains contains a mobile genetic element in the portion of glcK used to determine the ST of GBS strains. These two strains were found to be sequence type 61 (ST-61) after the inserted sequence was removed. The identical inserted sequence in the glcK gene has been found in other ST-61 bovine strains and is described elsewhere (21).

Main Article

1Presented in part at the XVth Lancefield International Symposium on Streptococci and Streptococcal Diseases, October 6–11, 2002, Goa, India.

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