Skip directly to site content Skip directly to page options Skip directly to A-Z link Skip directly to A-Z link Skip directly to A-Z link
Volume 13, Number 4—April 2007
Dispatch

16S rRNA Methylase–producing, Gram-Negative Pathogens, Japan

Kunikazu Yamane*, Jun-ichi Wachino*, Satowa Suzuki*, Naohiro Shibata*, Haru Kato*, Keigo Shibayama*, Kouji Kimura*, Kumiko Kai*, Satoshi Ishikawa*, Yoshiyuki Ozawa*, Toshifumi Konda*, and Yoshichika Arakawa*Comments to Author 
Author affiliations: *National Institute of Infectious Diseases, Tokyo, Japan;

Main Article

Figure 2

A) Pulsed-field gel electrophoresis (PFGE) fingerprinting patterns of SpeI-digested total DNA preparations from Pseudomonas aeruginosa. M, Lambda ladder PFGE molecular mass marker (Bio-Rad, Hercules, CA, USA). Strains 103 and 109 show similar patterns, which suggests probable nosocomial transmission of rmtA-positive strains in hospital C. Strains 113, 127, and 158 also demonstrate similar patterns, which implies possible nosocomial transmission in hospital D. However, 2 different PFGE patterns are observed in hospitals C, D, and F, which suggests transfer of plasmids carrying 16S rRNA–methylase genes among P. aeruginosa strains with different genetic backgrounds. B) SmaI-digested total DNA preparations from Acinetobacter baumannii isolated from hospital S. Three strains demonstrate the same PFGE pattern, which suggests probable nosocomial transmission of armA-positive A. baumannii in hospital S. M, lambda ladder low-range PFGE molecular mass marker (New England Biolabs, Ipswich, MA, USA).

Figure 2. A) Pulsed-field gel electrophoresis (PFGE) fingerprinting patterns of SpeI-digested total DNA preparations from Pseudomonas aeruginosa. M, Lambda ladder PFGE molecular mass marker (Bio-Rad, Hercules, CA, USA). Strains 103 and 109 show similar patterns, which suggests probable nosocomial transmission of rmtA-positive strains in hospital C. Strains 113, 127, and 158 also demonstrate similar patterns, which implies possible nosocomial transmission in hospital D. However, 2 different PFGE patterns are observed in hospitals C, D, and F, which suggests transfer of plasmids carrying 16S rRNA–methylase genes among P. aeruginosa strains with different genetic backgrounds. B) SmaI-digested total DNA preparations from Acinetobacter baumannii isolated from hospital S. Three strains demonstrate the same PFGE pattern, which suggests probable nosocomial transmission of armA-positive A. baumannii in hospital S. M, lambda ladder low-range PFGE molecular mass marker (New England Biolabs, Ipswich, MA, USA).

Main Article

Page created: June 25, 2010
Page updated: June 25, 2010
Page reviewed: June 25, 2010
The conclusions, findings, and opinions expressed by authors contributing to this journal do not necessarily reflect the official position of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.
file_external