Volume 13, Number 5—May 2007
Dispatch
Chikungunya Virus in US Travelers Returning from India, 2006
Table 1
Diagnostic test results for 35 travelers infected with chikungunya virus (CHIKV), 2006*
Sample | IgM ELISA† | IgG ELISA† | PRNT‡ | Virus isolation (Vero cells) | RT-PCR§ | Viremia, PFU/mL¶ | Days from onset of illness to collection | State of US residence | Return date, 2006 |
---|---|---|---|---|---|---|---|---|---|
1 |
17.7 |
3.2 |
640 |
– |
– |
NA |
0 |
NJ |
10/12 |
2 |
1.7 |
1.7 |
<10 |
– |
+ |
104.0 |
1 |
CA |
Before 11/28 |
3 |
1.2 |
1.1 |
ND |
+ |
+ |
104.1 |
1 |
IL |
9/29 |
4 |
1.8 |
NS |
ND |
+ |
+ |
106.8 |
2 |
CA |
Before 9/16 |
5 |
1.2 |
0.76 |
ND |
+ |
+ |
105.1 |
2 |
MA |
9/10 |
6 |
1.8 |
1.6 |
<10 |
+ |
+ |
106.0 |
3 |
PA |
Before 8/20 |
7 |
1.6 |
1.2 |
ND |
+ |
+ |
105.3 |
3 |
CA |
10/2 |
8 |
NS |
1.4 |
ND |
– |
+ |
103.9 |
4 |
WI |
10/9 |
9 |
22.0 |
4.3 |
5,120 |
– |
– |
NA |
4 |
CA |
Before 10/6 |
10 |
1.1 |
0.95 |
<10 |
– |
+ |
104.5 |
6 |
CA |
Before 8/13 |
11 |
7.4 |
0.96 |
40 |
– |
– |
NA |
7 |
CA |
Before 9/23 |
12 |
15.0 |
0.60 |
320 |
– |
– |
NA |
8 |
CT |
Before 7/6 |
13 |
26.2 |
1.2 |
160 |
ND |
– |
NA |
8 |
DC |
Before 10/16 |
14 |
12.9 |
5.8 |
1,80 |
ND |
– |
NA |
10 |
CA |
Before 9/22 |
15 |
38.8 |
2.3 |
2,560 |
ND |
ND |
NA |
19 |
CT |
Before 7/6 |
16 |
12.7 |
1.5 |
640 |
ND |
– |
NA |
20 |
IL |
8/23 |
17 |
16.9 |
4.8 |
640 |
ND |
– |
NA |
30 |
IL |
6/25 |
18 |
8.3 |
3.7 |
640 |
ND |
ND |
NA |
31 |
HI |
Before 8/2 |
19 |
6.6 |
1.5 |
640 |
ND |
– |
NA |
31 |
CA |
Before 8/13 |
20 |
2.6 |
6.8 |
320 |
ND |
– |
NA |
34 |
MD |
Before 3/20 |
21 |
5.0 |
NS |
640 |
ND |
– |
NA |
34 |
IL |
Before 9/22 |
22 |
15.1 |
4.1 |
1,280 |
ND |
– |
NA |
38 |
CA |
Before 10/2 |
23 |
4.3 |
5.9 |
2,560 |
ND |
– |
NA |
42 |
PA |
Before 8/20 |
24 |
5.6 |
NS |
320 |
ND |
– |
NA |
43 |
Unknown |
Unknown |
25 |
3.1 |
3.7 |
640 |
ND |
– |
NA |
44 |
IL |
Before 9/12 |
26 |
26.6 |
13.4 |
5,120 |
ND |
ND |
NA |
48 |
SC |
6/24 |
27 |
30.7 |
6.6 |
5,120 |
ND |
– |
NA |
61 |
CA |
Before 10/26 |
28 |
6.1 |
11.4 |
2,560 |
ND |
– |
NA |
62 |
CT |
Before 10/3 |
29 |
9.4 |
16.0 |
640 |
ND |
– |
NA |
63 |
CA |
8/23 |
30 |
7.5 |
8.4 |
1,280 |
ND |
– |
NA |
71 |
MN |
6/8 |
31 |
5.3 |
5.8 |
640 |
ND |
– |
NA |
71 |
MN |
6/8 |
32 |
3.7 |
16.1 |
320 |
ND |
– |
NA |
75 |
LA |
Before 3/30 |
33 |
9.8 |
10.1 |
2,560 |
ND |
– |
NA |
92 |
IL |
7/9 |
34 |
7.5 |
3.1 |
160 |
ND |
– |
NA |
101 |
PA |
Before 10/13 |
35 | 24.6 | 11.9 | 20,480 | ND | – | NA | Unknown | IL | Before 11/08 |
*IgM, immunoglobulin M; PRNT, plaque reduction neutralization test; RT-PCR, reverse transcription–PCR; NA, not applicable; ND, not done
(sampled depleted); NS, nonspecific reaction in ELISA.
†Values are patient sample optical densities divided by a negative control optical density; values >3 are positive.
‡Values are 90% plaque reduction neutralization titers.
§Real-time, fluorescence-based assay for detecting CHIKV RNA; positive samples had crossing threshold values <37 with both primer sets.
¶Estimated CHIKV PFU/mL by real-time RT-PCR using a standard curve generated with plaque-titrated/calibrated CHIKV standards.