Volume 14, Number 10—October 2008
Letter
Detecting Avian Influenza Virus (H5N1) in Domestic Duck Feathers
Yu Yamamoto
, Kikuyasu Nakamura, Masatoshi Okamatsu, Ayako Miyazaki, Manabu Yamada, and Masaji Mase
, Kikuyasu Nakamura, Masatoshi Okamatsu, Ayako Miyazaki, Manabu Yamada, and Masaji Mase
Table
Results of virus isolation and RT-PCR in 3 domestic ducks inoculated with influenza A virus (H5N1)*
| dpi | Virus titer (RT-PCR result)† |
||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Duck a |
Duck b |
Duck c |
|||||||||
| F | O | C | F | O | C | F | O | C | |||
| 2 | 4.2† (+)‡ | 2.0 (+) | – (–) | 1.7 (+) | 1.7 (+) | – (+) | 2.8 (+) | 2.0 (+) | 1.7 (–) | ||
| 3 | 6.9 (+) | 2.7 (+) | – (–) | 4.5 (+) | 3.0 (+) | 2.5 (+) | 4.8 (+) | 1.7 (+) | 2.8 (–) | ||
| 4 | 6.8 (+) | 3.7 (+) | 1.7 (–) | 2.3 (+) | 3.5 (+) | 1.7 (–) | 4.8 (+) | 3.3 (+) | – (–) | ||
| 5 | 6.5 (+) | – (+) | – (–) | 1.7 (+) | – (+) | – (–) | 2.5 (+) | 1.7 (+) | – (–) | ||
| 6 | 5.6 (+) | – (+) | – (–) | – (–) | – (–) | – (–) | 4.0 (+) | – (–) | – (–) | ||
| 7 | 3.8 (+) | – (–) | – (–) | – (–) | – (–) | – (–) | – (–) | – (–) | – (–) | ||
| 8 | 5.8 (+) | – (–) | – (–) | – (–) | – (–) | – (–) | – (+) | – (–) | – (–) | ||
| 9 | – (–) | – (–) | – (–) | – (–) | – (–) | – (–) | – (–) | – (–) | – (–) | ||
| 10 | – (–) | – (–) | – (–) | – (–) | – (–) | – (–) | – (–) | – (–) | – (–) | ||
*RT-PCR, reverse transcription–PCR; dpi, days postinoculation; F, feathers; O, oropharyngeal swabs; C, cloacal swabs.
†Virus titer expressed as 50% egg infectious doses per mL; –, negative for virus isolation. RT-PCR result: +, positive; –, negative.
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