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Volume 14, Number 12—December 2008
Dispatch

Novel Borna Virus in Psittacine Birds with Proventricular Dilatation Disease

Kirsi S. Honkavuori, H.L. Shivaprasad, Brent L. Williams, Phenix-Lan Quan, Mady Hornig, Craig Street, Gustavo Palacios, Stephen K. Hutchison, Monique Franca, Michael Egholm, Thomas BrieseComments to Author , and W. Ian Lipkin
Author affiliations: Columbia University, New York, NY, USA (K.S. Honkavuori, B.L. Williams, P.-L. Quan, M. Hornig, C. Street, G. Palacios, T. Briese, W.I. Lipkin); University of California Animal Health and Food Safety Laboratory System–Fresno Branch, Davis, California, USA (H.L. Shivaprasad, M. Franca); 454 Life Sciences, Branford, Connecticut, USA (S.K. Hutchison, M. Egholm)

Main Article

Table 1

Real-time PCR measurement of viral sequences in birds with PPD*

Case no. Species† Age/sex PDD/ control Primer/probe set Organ Ct Virus copies‡
(300 ng total RNA)
1034 Canindae macaw 30 y/M PDD 1034–1322 Brain 17.94 8.88 × 107
Adrenal gland 20.51 8.77 × 106
Proventriculus and gizzard
 25.26
 1.22 × 105
1322 Vinaceous Amazon 1 y/M PDD 1034–1322 Brain 17.64 1.17 × 108
Proventriculus
 28.47
 6.76 × 103
1367 Canindae macaw 30 y/F PDD 1367 Brain 27.33 8.89 × 103
Adrenal gland 18.92 3.87 × 106
Proventriculus
 23.82
 1.13 × 105
5473
 Leadbetter’s cockatoo
 17 y/M
 Control
 1034–1322 and 1367
 Brain
 >36§
 Negative
4858
 Long-tailed parakeet
 Adult/M
 Control
 1034–1322 and 1367
 Brain
 >36§
 Negative
2020
 Cockatiel
 Young/M
 Control
 1034–1322 and 1367
 Brain
 >36§
 Negative
3616 Eclectus parrot 0.25 y/F Control 1034–1322 and 1367 Brain >36§ Negative

*PPD, poventricular dilatation disease; Ct, cycle threshold.
†All birds were captive animals from California, USA.
‡Copy numbers were calculated on the basis of a standard curve generated from cloned target sequences.
§A Ct of >36 was rated as negative based on the highest dilution of standard representing 5 copies.

Main Article

Page created: July 22, 2010
Page updated: July 22, 2010
Page reviewed: July 22, 2010
The conclusions, findings, and opinions expressed by authors contributing to this journal do not necessarily reflect the official position of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.
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