Volume 14, Number 3—March 2008
Dispatch
Screening Pneumonia Patients for Mimivirus1
Ryan K. Dare*, Malinee Chittaganpitch†, and Dean D. Erdman*
Table 1
Primer and probe sequences for Acanthamoeba polyphaga mimivirus real-time PCR assays
| Target | Primer/probe | Gene region | Sequence (5′→3′) |
|---|---|---|---|
| APM-396 | Forward | ACC TGA TCC ACA TCC CAT AAC TAA A | |
| Reverse | Helicase | GGC CTC ATC AAC AAA TGG TTT C | |
| Probe | ACT CCA CCA CCT CCT TCT TCC ATA CCT TT | ||
| APM-596 | Forward | AAC AAT CGT CAT GGG AAT ATA GAA AT | |
| Reverse | Thiol oxidoreductase | CTT TCC AGT ATC CCT GTT CTT CAA | |
| Probe | TTC GTC ATA TGC GAG AAA ATG CTA TCC CT |
1Dare et al., Acanthamoeba polyphaga mimivirus real-time PCR assays, was presented at the 23rd Annual Clinical Virology Symposium, Apr 29–May 2, 2007, in Clearwater Beach, Florida, USA.
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