Volume 14, Number 4—April 2008
Research
Determination of Oseltamivir Quality by Colorimetric and Liquid Chromatographic Methods
Michael D. Green*
, Henry Nettey*, and Robert A. Wirtz*
, Henry Nettey*, and Robert A. Wirtz*
Table 1
Accuracy and precision for the high-performance liquid chromatographic (HPLC) and colorimetric assays (n = 5)
| Nominal concentration, mg/mL | Accuracy, % |
Precision, % |
|||
|---|---|---|---|---|---|
| Interday | Intraday | Interday | Intraday | ||
| HPLC | |||||
| 0.6 | –10.8 | –10.3 | 9.0 | 7.9 | |
| 1.5 | –8.4 | 0.4 | 7.3 | 2.3 | |
| 2.4 | 5.0 | –0.7 | 7.3 | 5.7 | |
| 3.0 | 0.4 | 1.6 | 4.9 | 4.8 | |
| 3.6 | 0.7 | 4.4 | 4.1 | 3.6 | |
| 4.5 |
–0.9 |
–3.2 |
2.5 |
2.5 |
|
| Congo red colorimetric | |||||
| 1.5 | 2.0 | 2.2 | 12.5 | 2.3 | |
| 2.4 | 2.6 | –0.3 | 9.2 | 6.9 | |
| 3.0 | –5.5 | 1.0 | 6.5 | 5.4 | |
| 3.6 | 0.7 | 1.2 | 3.7 | 3.8 | |
| 4.5 |
1.0 |
–0.1 |
2.6 |
1.3 |
|
| Bromochlorophenol blue colorimetric | |||||
| 6.0 | –5.3 | –19.4 | 18.6 | 12.4 | |
| 9.6 | 0.0 | 3.4 | 13.5 | 2.6 | |
| 12.0 | 2.7 | 10.4 | 6.1 | 5.9 | |
| 14.4 | 3.5 | 3.2 | 4.3 | 3.8 | |
| 18.0 | –2.9 | –5.4 | 4.9 | 3.0 | |
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