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Volume 15, Number 2—February 2009
Research

Severe Dengue Epidemics in Sri Lanka, 2003–2006

Nalaka Kanakaratne, Wahala M.P.B. Wahala, William B. Messer, Hasitha A. Tissera, Aruna Shahani, Nihal Abeysinghe, Aravinda M. de Silva, and Maya Gunasekera1
Author affiliations: Genetech Research Institute, Colombo, Sri Lanka (N. Kanakaratne, M. Gunasekera); University of North Carolina School of Medicine, Chapel Hill, North Carolina, USA (W.M.P.B. Wahala, W.B. Messer, A.M de Silva); Ministry of Health, Colombo (H.A. Tissera, N. Abeysinghe); Apollo Hospital, Colombo (A. Shahani)

Main Article

Figure 5

Phylogram of dengue serotype 2 viruses (DENV-2) from Sri Lanka (SL), 1981–2004, and other DENV-2 viruses. The tree is based on a 239-bp fragment for positions 2311–2550 coding for amino acids at the envelope protein/nonstructural protein 1 junction. The tree was constructed as described in Figure 4 and was rooted by using a DENV-2 sylvatic strain. Classification and naming of different DENV-2 genotypes is based on the report by Rico-Hesse (5). Scale bar represents number of base substitutions pe

Figure 5. Phylogram of dengue serotype 2 viruses (DENV-2) from Sri Lanka (SL), 1981–2004, and other DENV-2 viruses. The tree is based on a 239-bp fragment for positions 2311–2550 coding for amino acids at the envelope protein/nonstructural protein 1 junction. The tree was constructed as described in Figure 4 and was rooted by using a DENV-2 sylvatic strain. Classification and naming of different DENV-2 genotypes is based on the report by Rico-Hesse (5). Scale bar represents number of base substitutions per site.

Main Article

References
  1. Mackenzie  JS, Gubler  DJ, Petersen  LR. Emerging flaviviruses: the spread and resurgence of Japanese encephalitis, West Nile and dengue viruses. Nat Med. 2004;10(Suppl):S98–109. DOIPubMedGoogle Scholar
  2. Innis  BL. Antibody responses to dengue virus infection. In: Gubler DJ, Kuno G, editors. Dengue and dengue hemorrhagic fever. New York: CAB International; 1997. p. 221–44.
  3. Halstead  SB. Neutralization and antibody-dependent enhancement of dengue viruses. Adv Virus Res. 2003;60:421–67. DOIPubMedGoogle Scholar
  4. Rothman  AL. Dengue: defining protective versus pathologic immunity. J Clin Invest. 2004;113:946–51.PubMedGoogle Scholar
  5. Rico-Hesse  R. Microevolution and virulence of dengue viruses. Adv Virus Res. 2003;59:315–41. DOIPubMedGoogle Scholar
  6. Leitmeyer  KC, Vaughn  DW, Watts  DM, Salas  R, Villalobos  I, de Chacon, et al. Dengue virus structural differences that correlate with pathogenesis. J Virol. 1999;73:4738–47.PubMedGoogle Scholar
  7. Messer  WB, Gubler  DG, Harris  E, Sivananthan  K, de Silva  AM. Emergence and global spread of a dengue serotype 3, subtype III virus. Emerg Infect Dis. 2003;9:800–9.PubMedGoogle Scholar
  8. Messer  WB, Vitarana  UT, Sivananthan  K, Elvtigala  J, Preethimala  LD, Ramesh  R, Epidemiology of dengue in Sri Lanka before and after the emergence of epidemic dengue hemorrhagic fever. Am J Trop Med Hyg. 2002;66:765–73.PubMedGoogle Scholar
  9. Vitarana  UT, Jayasekera  N, Withane  N, Gubler  DJ. Finding the cause of dengue hemorrhagic fever outbreaks in Sri Lanka. Arbovirus Research in Australia. 1993;6:125–9.
  10. Seah  CL, Chow  VT, Tan  HC, Can  YC. Rapid, single-step RT-PCR typing of dengue viruses using five NS3 gene primers. J Virol Methods. 1995;51:193–200. DOIPubMedGoogle Scholar
  11. Sudiro  TM, Ishiko  H, Green  S, Vaughn  DW, Nisalak  A, Kalayanarooj  S, Rapid diagnosis of dengue viremia by reverse transcriptase-polymerase chain reaction using 3′-noncoding region universal primers. Am J Trop Med Hyg. 1997;56:424–9.PubMedGoogle Scholar
  12. Rzhetsky  A, Nei  M. AMETREE: a program package for inferring and testing minimum-evolution trees. Comput Appl Biosci. 1994;10:409–12.PubMedGoogle Scholar
  13. Felsenstein  J. Confidence limits on phylogenies: an approach using the bootstrap. Evolution Int J Org Evolution. 1985;39:783–91. DOIGoogle Scholar
  14. Tamura  K, Dudley  J, Nei  M, Kumar  S. MEGA4: molecular evolutionary genetics analysis (MEGA) software version 4.0. Mol Biol Evol. 2007;24:1596–9. DOIPubMedGoogle Scholar
  15. Lanciotti  RS, Lewis  JG, Gubler  DJ, Trent  DW. Molecular evolution and epidemiology of dengue-3 viruses. J Gen Virol. 1994;75:65–75. DOIPubMedGoogle Scholar
  16. Carey  DE, Myers  RM, Reuben  R. Dengue types 1 and 4 viruses in wild-caught mosquitoes in south India. Science. 1964;143:131–2. DOIPubMedGoogle Scholar
  17. Myers  RM, Varkey  MJ, Reuben  R, Jesudass  ES. Dengue outbreak in Vellore, southern India, in 1968, with isolation of four dengue types from man and mosquitoes. Indian J Med Res. 1970;58:24–30.PubMedGoogle Scholar
  18. Vitarana  T. Viral diseases in Sri Lanka: a national overview. In: Mackenzie JS, editor. Viral diseases in Southeast Asia and the western Pacific London: Academic Press; 1982. p. 198–204.
  19. Rahman  M, Rahman  K, Siddque  AK, Shoma  S, Kamal  AH, Ali  KS, First outbreak of dengue hemorrhagic fever, Bangladesh. Emerg Infect Dis. 2002;8:738–40.PubMedGoogle Scholar
  20. Chan  YC, Salahuddin  NI, Khan  J, Tan  HC, Seah  CL, Li  J, Dengue haemorrhagic fever outbreak in Karachi, Pakistan, 1994. Trans R Soc Trop Med Hyg. 1995;89:619–20. DOIPubMedGoogle Scholar
  21. Bharaj  P, Chahar  HS, Pandey  A, Diddi  K, Dar  L, Guleria  R, Concurrent infections by all four dengue virus serotypes during an outbreak of dengue in 2006 in Delhi, India. Virol J. 2008;5:1. DOIPubMedGoogle Scholar
  22. Dar  L, Broor  S, Sengupta  S, Xess  I, Seth  P. The first major outbreak of dengue hemorrhagic fever in Delhi, India. Emerg Infect Dis. 1999;5:589–90.PubMedGoogle Scholar
  23. Lucas  GN, Amerasinghe  A, Sriranganathan  S. Dengue haemorrhagic fever in Sri Lanka. Indian J Pediatr. 2000;67:503–4. DOIPubMedGoogle Scholar
  24. Hanley  KA, Nelson  JT, Schirtzinger  EE, Whitehead  SS, Hanson  CT. Superior infectivity for mosquito vectors contributes to competitive displacement among strains of dengue virus. BMC Ecol. 2008;8:1. DOIPubMedGoogle Scholar
  25. Bennett  SN, Holmes  EC, Chirivella  M, Rodriguez  DM, Beltran  M, Vorndam  V, Selection-driven evolution of emergent dengue virus. Mol Biol Evol. 2003;20:1650–8. DOIPubMedGoogle Scholar
  26. Bennett  SN, Holmes  EC, Chirivella  M, Rodriguez  DM, Beltran  M, Vorndam  V, Molecular evolution of dengue 2 virus in Puerto Rico: positive selection in the viral envelope accompanies clade reintroduction. J Gen Virol. 2006;87:885–93. DOIPubMedGoogle Scholar

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1Deceased.

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