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Volume 15, Number 3—March 2009
Research

Shiga Toxin–producing Escherichia coli Strains Negative for Locus of Enterocyte Effacement

Hayley J. Newton1, Joan Sloan1, Dieter M. Bulach, Torsten Seemann, Cody C. Allison, Marija Tauschek, Roy M. Robins-Browne, James C. Paton, Thomas S. Whittam, Adrienne W. Paton, and Elizabeth L. HartlandComments to Author 
Author affiliations: Monash University, Melbourne, Victoria, Australia (H.J. Newton, J. Sloan, D.M. Bulach, T. Seemann, C.C. Allison, E.L. Hartland); University of Melbourne, Melbourne (H.J. Newton, J. Sloan, M. Tauschek, R.M. Robins-Browne, E.L. Hartland); University of Adelaide, Adelaide, South Australia, Australia (J.C. Paton, A.W. Paton); Michigan State University, East Lansing, Michigan, USA (T.S. Whittam)

Main Article

Table 2

Gene names, plasmid location, and oligonucleotide sequences used to examine the prevalence of pO113-encoded genes among STEC strains*

Gene Plasmid location, bp Oligonucleotide sequences Amplicon size, bp PCR annealing temperature, °C
pilQ 9593–12105 F: 5’-TTGCAGACCCGCAGTTG-3’
R: 3’-CAGGGCTTCGGCGATGT-5’ 870 52
epeA 48716–52795 F:5’-CAGGTGGTACTGTCGGC-3’
R: 3’-GCCCATGCCGCTCTGAA-5’ 667 46
trbC 57357–59660 F: 5’-GCCACCACCGGTGGCGG-3’
R: 3’-CAATCAGAATGCGGTCG-5’ 230 50
repA 106112–107089 F: 5’-AAAGTCTTGTATAGCTC-3’
R: 3’-GTTATCCATATCCAGGC-5’ 871 44
ehxA 114136–117132 F: 5’-CCCAGGAGAAGAAGTCA-3’
R: 3’-CTTCACCTGAGGCATCTT-5’ 1,108 48
espP 134205–140707 F: 5’-AAACAGCAGGCACTTGAACG-3’
R: 3’-GGAGTCGTCAGTCAGTAGAT-5’ 2,000 52
iha 146066–149549 F: 5’-TCCAGTCAGTACCACGA-3’
R: 3’-CTGTCGGAAAGTTTCAC-5’ 981 48
subAB 150678–152419 F: 5’-GTGTACAGGACTCATGG-3’
R: 3’-ATCACCAGTCCACTCAG-5’ 783 48
repZ 163918–164949 F: 5’-ATACAGGAGTAAAACCG-3’
R: 3’-CATATAACGCAGTACAC-5’ 1,792 46

*STEC, Shiga toxin–producing Escherichia coli; F, forward primer; R, reverse primer.

Main Article

1These authors contributed equally to this article.

Page created: December 07, 2010
Page updated: December 07, 2010
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