Volume 15, Number 5—May 2009
Research
Chronic Wasting Disease Prions in Elk Antler Velvet
Figure 6
![Detection of CerPrPSc (disease-associated form of cervid prion protein [PrP]) in brain and antler velvet from chronic wasting disease (CWD)–affected elk after serial protein misfolding cyclic amplification (PMCA). Western blots demonstrate amplification of protease-resistant prion protein (PrP) after serial PMCA when seeded with brain or velvet antler material from CWD-affected elk. Brain samples: lane 1, Tg(CerPrP)1536+/– brain material not treated with proteinase K (PK); lane 2, Tg(CerPrP)1536](/eid/images/08-1458-F6.jpg)
Figure 6. Detection of CerPrPSc (disease-associated form of cervid prion protein [PrP]) in brain and antler velvet from chronic wasting disease (CWD)–affected elk after serial protein misfolding cyclic amplification (PMCA). Western blots demonstrate amplification of protease-resistant prion protein (PrP) after serial PMCA when seeded with brain or velvet antler material from CWD-affected elk. Brain samples: lane 1, Tg(CerPrP)1536+/– brain material not treated with proteinase K (PK); lane 2, Tg(CerPrP)1536+/– brain material used as a negative control seed for the PMCA reactions; lanes 3–9, 10–2 to 10–8 dilutions of 10% elk brain homogenate. Antler velvet samples: lane 1, Tg(CerPrP)1536+/– brain material not treated with PK; lane 2, Tg(CerPrP)1536+/– brain material used as a negative control seed for the PMCA reactions; lanes 3–9, replicate 10–2 dilutions of 10% antler velvet homogenate. Samples were either treated or not treated with PK as indicated. Membranes were probed with monoclonal antibody 6H4. Tg, transgenic.