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Volume 15, Number 8—August 2009
Research

Reproducibility of Serologic Assays for Influenza Virus A (H5N1)

Iain StephensonComments to Author , Alan Heath, Diane Major, Robert W. Newman, Katja Hoschler, Wang Junzi, Jacqueline M. Katz, Jerry P. Weir, Maria C. Zambon, and John M. Wood
Author affiliations: University of Leicester, Leicester, UK (I. Stephenson); National Institute for Biological Standards and Controls, Potters Bar, UK (A. Heath, D. Major, R.W. Newman, J.M. Wood); Health Protection Agency, Colindale, UK (K. Hoschler, M.C. Zambon); Centers for Disease Control and Prevention, Atlanta, Georgia, USA (J.M. Katz); Food and Drug Administration, Rockville, Maryland, USA (J.P. Weir); National Institute for the Control of Pharmaceutical and Biological Products, Beijing, People’s Republic of China (W. Junzhi)

Main Article

Figure 1

Intralaboratory reproducibility showing proportion (%) of replicate assays differing by >2-fold (A, B) and >4-fold (C, D) by horse hemagglutination-inhibition and neutralization assays for each participating laboratory for all serum samples (A, C) and postvaccination serum samples (B, D). Laboratory 11 did not return hemagglutinin-inhibition result.

Figure 1. Intralaboratory reproducibility showing proportion (%) of replicate assays differing by >2-fold (A, B) and >4-fold (C, D) by horse hemagglutination-inhibition and neutralization assays for each participating laboratory for all serum samples (A, C) and postvaccination serum samples (B, D). Laboratory 11 did not return hemagglutinin-inhibition result.

Main Article

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