Volume 15, Number 8—August 2009
Dispatch
Legionella pneumophila in Rainwater on Roads
Ryota Sakamoto, Akira Ohno
, Toshitaka Nakahara, Kazunari Satomura, Suketaka Iwanaga, Yuuichiro Kouyama, Fumiaki Kura, Naoyuki Kato, Kozo Matsubayashi, Kiyohito Okumiya, and Keizo Yamaguchi
, Toshitaka Nakahara, Kazunari Satomura, Suketaka Iwanaga, Yuuichiro Kouyama, Fumiaki Kura, Naoyuki Kato, Kozo Matsubayashi, Kiyohito Okumiya, and Keizo Yamaguchi
Table
Legionella spp. and Acanthamoeba spp. isolated from asphalt road sources, Tokyo, Japan, July–October 2007
| Source | Positive samples, no. (%) |
|---|---|
| Primary survey | |
| Puddles on roads (n = 18) | |
| L. pneumophila* | 7 (38.9) |
| Acanthamoeba spp.† | 4 (22.2) |
| Rainwater (n = 10) | |
| L. pneumophila* | 0 |
| Legionella spp.‡ | 1 (10.0) |
| Acanthamoeba spp.† | 3 (30.0) |
| Swabs from roads on sunny days (n = 12) | |
| L. pneumophila* | 0 |
| Legionella spp.‡ | 2 (16.7) |
| Acanthamoeba spp.† | 2 (16.7) |
| Fixed-point observation of puddles on roads | |
| L. pneumophila concentration (n = 45) | |
| <20 CFU/100 mL | 29 (64.4) |
| 20–99 CFU/100 mL | 7 (15.6) |
| 100–999 CFU/100 mL | 7 (15.6) |
| >1,000 CFU/100 mL | 2 ( 4.4) |
| L. pneumophila serogroups (n = 150 strains isolated) | |
| 1 | 56 (37.3) |
| 3 | 50 (33.3) |
| 2 | 15 (10.0) |
| 6 | 12 ( 8.0) |
| 5 | 11 ( 7.3) |
| Others | 6 ( 4.0) |
*Tested by using culture method.
†Tested by using PCR method.
‡Tested by using loop-mediated isothermal amplification method.
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