Volume 16, Number 4—April 2010
Dispatch
Novel Corynebacterium diphtheriae in Domestic Cats
Table
Strain | Culture collection | Source | Diphtheria toxin | GenBank accession no. |
||
---|---|---|---|---|---|---|
16S rRNA | rpoB | tox gene | ||||
CD443 | ATCC BAA-1774 | Cat 1, right ear | Nontoxigenic | FJ409572 | FJ415317 | FJ376656 |
CD448 | ND | Cat 1, right ear | Nontoxigenic | FJ409573 | ND | FJ422272 |
CD449 | ND | Cat 1, left ear | Nontoxigenic | FJ409574 | ND | FJ422273 |
CD450 | ND | Cat 2, left ear | Nontoxigenic | FJ409575 | FJ415318 | FJ422274 |
C. diphtheriae biotype mitis | NCTC 10356† | Human nose | Nontoxigenic | GQ118340 | GQ409648 | ND |
C. diphtheriae biotype gravis | NCTC 10648 | Unknown | Toxigenic | ND | ND | ND |
C. diphtheriae biotype gravis | NCTC 11397T ATCC 27010T | Unknown | Nontoxigenic | GQ118341 | GQ409649 | ND |
C. diphtheriae biotype gravis | NCTC 13129 ATCC 700971 | Human throat | Unknown | GQ118344 | GQ409650 | ND |
C. pseudotuberculosis | NCTC 3450T | Sheep gland | Unknown | GQ118342 | GQ409651 | ND |
C. ulcerans | NCTC 12077 | Human throat | Unknown | GQ118343 | ND | ND |
C. ulcerans | NCTC 7910 | Human throat | Unknown | GQ118345 | ND | ND |
*CD, Centers for Disease Control and Prevention identifier number; ATCC, American Type Culture Collection; ND, not deposited in this study; NCTC, National Collection of Type Cultures, London, UK. Additional strains used as controls for specific assays: toxigenic C. diphtheriae biotype belfanti isolates used for real-time PCR of tox gene were 718, G4182, C59, C60, C75, C76, C77; toxigenic C. diphtheriae ATCC 27012 used as positive control for Elek; C. diphtheriae NCTC 10481 and C. ulcerans CD199 used as positive and negative controls for Vero cell assay.
†NCTC 10356 is described in the NCTC catalogue as C. diphtheriae biotype mitis; however, analyses in this study found this strain to be nitrate negative and therefore consistent with C. diphtheriae biotype belfanti. Thus, it was used in this study as a belfanti reference strain.