Volume 6, Number 1—February 2000
Dispatch
Molecular Genetic Evidence of a Novel Morbillivirus in a Long-Finned Pilot Whale (Globicephalus melas)
Figure 2

Figure 2. Neighbor-joining analyses of partial P and N gene sequences with branch distances as shown. Analyses were performed with MEGA, version 1.01 (13). For the P gene, a 378 nucleotide fragment was amplified (7,8) using the following primers: 5'-CGGAG ACCGAGTCTTCATT-3' (forward) and 5'-ATTGGGTTGC ACCACTTG TC-3' (reverse), corresponding to nucleotides 2190 to 2567 as aligned to the measles virus P gene (Edmonston strain). For the N gene, a 230-nucleotide fragment was amplified using the following primers: 5'-CCHAGRATYGCTGAAATGATHTGTGA-3' (forward) and 5'-AACTTG TTCTGRATWGAGTTYTC-3' (reverse), corresponding to nucleotides 849 to 1078, as aligned to the measles virus N gene (Edmonston strain, GenBank accession number Z66517). RT-PCR and sequence analysis were performed as described (7,8).
References
- Kennedy S. Morbillivirus infections in aquatic mammals. J Comp Pathol. 1998;119:201–25. DOIPubMedGoogle Scholar
- Lipscomb TP, Schulman FY, Moffett D, Kennedy S. Morbilliviral disease in Atlantic bottlenose dolphins (Tursiops truncatus) from the 1987-1988 epizootic. J Wildl Dis. 1994;30:567–71.PubMedGoogle Scholar
- Schulman FY, Lipscomb TP, Moffett D, Krafft AE, Lichy JH, Tsai MM, Reevaluation of the 1987-88 Atlantic coast bottlenose dolphin (Tursiops truncatus) mortality event with histologic, immunohistochemical, and molecular evidence for a morbilliviral etiology. Vet Pathol. 1997;34:288–95. DOIPubMedGoogle Scholar
- Osterhaus ADME, Groen J, Spijkers HEM, Broeders HWJ, UytdeHaag FGCM, de Vries P, . Mass mortality in seals caused by a newly discovered morbillivirus. Vet Microbiol. 1990;23:343–50. DOIPubMedGoogle Scholar
- Kennedy S, Smyth JA, Cush PF, McAliskey M, McCullough SJ, Rima BK. Seven histopathologic and immunocytochemical studies of distemper in harbor porpoises. Vet Pathol. 1991;28:1–7. DOIPubMedGoogle Scholar
- Domingo M, Vilafranca M, Visa J, Prats N, Trudgett AL, Visser I. Evidence of chronic morbillivirus infection in the Mediterranean striped dolphin (Stenella coeruleoalba). Vet Microbiol. 1995;44:229–39. DOIPubMedGoogle Scholar
- Reidarson TH, McBain J, House C, King DP, Stott JL, Krafft AE, Morbillivirus infection in stranded common dolphins (Delphinus delphis) from the Pacific Ocean. J Wildl Dis. 1998;34:771–6.PubMedGoogle Scholar
- Barrett T, Visser IKG, Mamaev L, Goatley L, van Bressem M-F, Osterhaus ADME. Dolphin and porpoise morbilliviruses are genetically distinct from phocine distemper virus. Virology. 1993;193:1010–2. DOIPubMedGoogle Scholar
- Taubenberger JK, Tsai MM, Krafft AE, Lichy JH, Reid AH, Schulman FY, Two different morbilliviruses implicated in bottlenose dolphin epizootics. Emerg Infect Dis. 1996;2:213–6. DOIPubMedGoogle Scholar
- Swofford DL. PAUP: Phylogenetic Analysis Using Parsimony, version 3.1.1 (Illinois Natural History Survey, Champaign, Illinois; 1991.
- Duignan PJ, House C, Geraci JR, Duffy N, Rima BK, Walsh MT, Morbillivirus infection in cetaceans of the western Atlantic. Vet Microbiol. 1995;44:241–9. DOIPubMedGoogle Scholar
- Duignan PJ, House C, Geraci JR, Early G, Copland HG, Walsh MT, Morbillivirus infection in two species of pilot whales (Globicephala sp.) from the western Atlantic. Mar Mamm Sci. 1995;11:150–62. DOIGoogle Scholar