Volume 8, Number 4—April 2002
Research
Genetic and Antigenic Analysis of the First A/New Caledonia/20/99-like H1N1 Influenza Isolates Reported in the Americas
Luke T. Daum*, Linda C. Canas*, Catherine B. Smith†, Alexander Klimov†, William Huff*, William Barnes*, and Kenton L. Lohman*
Table 1
Characterization of Influenza A H1N1 (HA1) Sequencing Primers
| Primer/nt position | Sequence | % GCa | Tmb(°C) |
|---|---|---|---|
| H1F-272 | 5′-AATCATGGTCCTACATTG-3′ | 38.9 | 57 |
| H1F-734 | 5′-ACTACTACTGGACTCTGC-3′ | 50 | 58 |
| H1R-365 | 5′-TTCCTCATACTCGGCGAA-3′ | 50 | 58 |
| H1R-1110 | 5′-CCATCCATCTATCATTCC-3′ | 44.4 | 55 |
aGC= (Guanosine (G) + Cytidine (C))/ (Adenosine (A)+ Thymidine (T)+ Guanosine (G) + Cytidine (C)) x 100
bTm= 81.5 + 16.6 * log [Na+] + 41 * (#G + #C)/length – 500/length where [Na+]= 0.1M.
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