Volume 9, Number 7—July 2003
Research
Amoeba-Resisting Bacteria and Ventilator-Associated Pneumonia
Bernard La Scola*, Ioanna Boyadjiev*†, Gilbert Greub*, Atieh Khamis*, Claude Martin†, and Didier Raoult*†
Table 2
Polymerase chain reaction primers used for amplification and sequencing of bacterial DNA within human samples
| Primers | Bosea-related strain | Legionella anisa | Afipia broomeae | L. quinlivanii |
|---|---|---|---|---|
| External forward |
5′-TGCGAGTGTAGAGGTGAAATT-3′ |
5′-TATTGGTGCTGATTTAGGAA-3′ |
5′-TCTTTTGTGCGGGAAGATAATG-3′ |
5′-TTGTTGATGTTTGTTTTGAGACC-3′ |
| External reverse |
5′CGCTCGTTGCGGGACTTAA-3′ |
5′-GCTAAGTCTGAAGGTACA-3′ |
5′-TAAACTTTCCAACGGCTGGCAT-3′ |
5′-TTCAACACTTCTTTCATCTGATC-3′ |
| Internal forward |
5′-GAGGTGAAATTCGTAGATATT-3′ |
5′-GCCCAATTGATTTTGACAG-3′ |
5′-GCTAACTTCGTGCCAGCAG-3′ |
5′-TCCAAGAATAAAAGGGGATTG-3′ |
| Internal reverse | 5′-GAGCTGACGACAGCCAT-3′ | 5′-GCATTAATTGTAATGCTTCA-3′ | 5′-GTTTGCTCCCCACGCTTTC-3′ | 5′-CCATACCATCCTGTAAGCCTT-3′ |
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