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Volume 9, Number 8—August 2003

Research

Molecular Analysis of Echovirus 13 Isolates and Aseptic Meningitis, Spain

Ana Avellón*Comments to Author , Inmaculada Casas*, Gloria Trallero*, Carmen Pérez†, Antonio Tenorio*, and Gustavo Palacios‡
Author affiliations: *Instituto de Salud Carlos III, Madrid, Spain; †Doctor Negrín Hospital, Las Palmas de Gran Canaria, Canary Island; ‡Columbia University, New York, New York, USA

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Figure 2

Phylogenetic tree of the VP1 3′ terminal region, which identifies the Spanish isolates as echovirus 13 (EV13) and differentiates two clusters (Iberian Peninsula and Canary Islands). The sequences included are, apart from the reference strains, all the Spanish EV13 (61 isolates and 3 sequences obtained directly from cerebrospinal fluid) and two American isolates. Model of nucleotide substitution: Kimura two parameters. Phylogenetic tree reconstructed with the neighbor joining method, and bootstra

Figure 2. Phylogenetic tree of the VP1 3′ terminal region, which identifies the Spanish isolates as echovirus 13 (EV13) and differentiates two clusters (Iberian Peninsula and Canary Islands). The sequences included are, apart from the reference strains, all the Spanish EV13 (61 isolates and 3 sequences obtained directly from cerebrospinal fluid) and two American isolates. Model of nucleotide substitution: Kimura two parameters. Phylogenetic tree reconstructed with the neighbor joining method, and bootstrap analysis with 1,000 pseudoreplicate datasets.

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