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Volume 1, Number 3—July 1995

Dispatch

HIV-1 Patients May Harbor Viruses of Different Phylogenetic Subtypes: Implications for the Evolution of the HIV/AIDS Pandemic

Danuta Pieniazek*, Luiz M. Janini*#, Artur Ramos*#, Amilcar Tanuri#, Mauro Schechter, Jose M. Peralta#, Anna C.P. Vicente§, Norman J. Pieniazek*, Gerald Schochetman*, and Mark A. Rayfield*
Author affiliations: *National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia, USA; 
#Universidade Federal do Rio de Janeiro, and Hospital Clementino Fraga Filho; §Instituto Oswaldo Cruz, Rio de Janeiro, Brazil

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Figure 1

A. Three distinct A1uI digestion patterns of PCR amplified protease gene representing single HIV-1 infections by viral strains of subtypes A, C, F (pattern #1), and subtypes B and D (patterns #2 and #3). B. The presence of two distinct A1uI digestion patterns (#1 and #2) of the protease gene in PBMC of the patient dually infected by viral strains of subtypes F and B (lane 3). Arrows indicate diagnostic fragments deteted by hybridization with the radioactive probe (2). MW represents molecular wei

Figure 1. A. Three distinct A1uI digestion patterns of PCR amplified protease gene representing single HIV-1 infections by viral strains of subtypes A, C, F (pattern #1), and subtypes B and D (patterns #2 and #3). B. The presence of two distinct A1uI digestion patterns (#1 and #2) of the protease gene in PBMC of the patient dually infected by viral strains of subtypes F and B (lane 3). Arrows indicate diagnostic fragments deteted by hybridization with the radioactive probe (2). MW represents molecular weight markers-Ø RF DNA, HaeIII digest.

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