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Volume 10, Number 10—October 2004
Research

Fluoroquinolone Resistance in Penicillin-resistant Streptococcus pneumoniae Clones, Spain

Adela G. de la Campa*Comments to Author , Luz Balsalobre*, Carmen Ardanuy†, Asunción Fenoll*, Emilio Pérez-Trallero‡, Josefina Liñares†, and the Spanish Pneumococcal Infection Study Network G03/103
Author affiliations: *Instituto de Salud Carlos III, Majadahonda, Madrid, Spain; †Hospital de Bellvitge, L’Hospitalet de Llobregat, Barcelona, Spain; ‡Hospital Donostia, San Sebastian, Guipúzcoa, Spain

Main Article

Table 2

Fluoroquinolone MIC of 85 strains and amino acid changes in their DNA topoisomerase genesa

No. strains Amino acid substitution
MIC (μg/mL)
ParC Par E GyrA CIP LVX GAT MXF GEMI
10 None None None 1–2 1–2 0.12–0.5 0.12–0.25 0.015–0.03
1 None None None 8 2 0.5 0.12 0.06
8 S79F None None 4–8 2–4 0.5–2 0.12–0.5 0.03–0.06
3 S79Y None None 4–8 2–4 0.5 0.25 0.03–0.06
1 D83G None None 4 1 0.5 0.25 0.03
1 D83Y None None 4 1 0.5 0.25 0.03
1 S79A None S81F 16 8 4 2 0.12
21 S79F None S81F 32–128 8–32 4–8 2–4 0.12–1
9 S79F None S81Y 32–64 8–16 4–8 1–4 0.12–0.25
6 S79F None E85K 32–64 16–64 4–16 2–8 0.25–2
6 S79Y None S81F 32–64 8–64 4–8 2–4 0.12–0.25
1 S79Y None S81Y 32 16 8 4 0.12
2 D83N None S81F 32–64 8–16 2–4 2 0.12–0.25
1 D83N None S81F 32 16 8 4 0.5
3 None D435N S81F 16–32 8–16 4–8 2–4 0.12–0.25
1 None D435N S81Y 16 16 4 2 0.12
1 S79Y, D83N None E85K 64 32 8 4 1
3 S79F D435N E85K 64 64 8–16 8 0.5
1 S79Y D435N E85K 64 32 8 4 0.5
1 S79F None S81F, E85A 64 64 16 8 1
1 S79I None S81F 32 16 4 2 0.12
1 S79F None S81F 32 16 4 4 0.5
1 S79F None S81F 32 8 4 2 0.25
1 S79R None S81F 32 16 8 4 0.5

aOnly changes involved in resistance are shown, and double underlining indicates that the residue is located in a gene with a mosaic structure. Additional amino acid changes, not involved in resistance, were: ParC D78A (the CipR-71 strain with no mutations that has a mosaic parC gene), R36C (3 strains), ParC K137N (27 strains), ParC N91D (the three strains with mosaic parC genes), ParE I460V (40 strains), ParE453S (1 strain), GyrA V88I (3 strains), GyrA S114G (the two strains with mosaic gyrA genes), and GyrA N150H (one of the two strains with a mosaic gyrA gene). CIP, ciprofloxacin; LVX, levofloxacin; GAT, gatifloxacin; MXF, moxifloxacin; GEMI, gemifloxacin.

Main Article

1Spanish Pneumococcal Infection Study Network—general coordination: Román Pallarés; participants and centers: Ernesto García (Centro de Investigaciones Biológicas, Madrid); Julio Casal, Asuncion Fenoll, Adela G. de la Campa (Centro Nacional de Microbiología, Instituto de Salud Carlos III, Madrid); Emilio Bouza, (Hospital Gregorio Marañon, Madrid); Fernando Baquero (Hospital Ramón y Cajal, Madrid); Francisco Soriano, José Prieto (Fundación Jiménez Díaz y Hospital Clínico, Madrid); Román Pallarés, Josefina Liñares (Hospital Universitari de Bellvitge, Barcelona); Javier Garau, Javier Martínez Lacasa (Hospital Mutua de Terrassa, Barcelona); Cristina Latorre (Hospital Sant Joan de Deu, Barcelona); Emilio Pérez-Trallero, Alberto González (Hospital Donostia, San Sebastian); Juan García de Lomas (Hospital Clínico, Valencia); and Ana Fleites (Hospital Central de Asturias).

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