Volume 10, Number 10—October 2004
Research
Fluoroquinolone Resistance in Penicillin-resistant Streptococcus pneumoniae Clones, Spain
Table 2
No. strains | Amino acid substitution |
MIC (μg/mL) |
||||||
---|---|---|---|---|---|---|---|---|
ParC | Par E | GyrA | CIP | LVX | GAT | MXF | GEMI | |
10 | None | None | None | 1–2 | 1–2 | 0.12–0.5 | 0.12–0.25 | 0.015–0.03 |
1 | None | None | None | 8 | 2 | 0.5 | 0.12 | 0.06 |
8 | S79F | None | None | 4–8 | 2–4 | 0.5–2 | 0.12–0.5 | 0.03–0.06 |
3 | S79Y | None | None | 4–8 | 2–4 | 0.5 | 0.25 | 0.03–0.06 |
1 | D83G | None | None | 4 | 1 | 0.5 | 0.25 | 0.03 |
1 | D83Y | None | None | 4 | 1 | 0.5 | 0.25 | 0.03 |
1 | S79A | None | S81F | 16 | 8 | 4 | 2 | 0.12 |
21 | S79F | None | S81F | 32–128 | 8–32 | 4–8 | 2–4 | 0.12–1 |
9 | S79F | None | S81Y | 32–64 | 8–16 | 4–8 | 1–4 | 0.12–0.25 |
6 | S79F | None | E85K | 32–64 | 16–64 | 4–16 | 2–8 | 0.25–2 |
6 | S79Y | None | S81F | 32–64 | 8–64 | 4–8 | 2–4 | 0.12–0.25 |
1 | S79Y | None | S81Y | 32 | 16 | 8 | 4 | 0.12 |
2 | D83N | None | S81F | 32–64 | 8–16 | 2–4 | 2 | 0.12–0.25 |
1 | D83N | None | S81F | 32 | 16 | 8 | 4 | 0.5 |
3 | None | D435N | S81F | 16–32 | 8–16 | 4–8 | 2–4 | 0.12–0.25 |
1 | None | D435N | S81Y | 16 | 16 | 4 | 2 | 0.12 |
1 | S79Y, D83N | None | E85K | 64 | 32 | 8 | 4 | 1 |
3 | S79F | D435N | E85K | 64 | 64 | 8–16 | 8 | 0.5 |
1 | S79Y | D435N | E85K | 64 | 32 | 8 | 4 | 0.5 |
1 | S79F | None | S81F, E85A | 64 | 64 | 16 | 8 | 1 |
1 | S79I | None | S81F | 32 | 16 | 4 | 2 | 0.12 |
1 | S79F | None | S81F | 32 | 16 | 4 | 4 | 0.5 |
1 | S79F | None | S81F | 32 | 8 | 4 | 2 | 0.25 |
1 | S79R | None | S81F | 32 | 16 | 8 | 4 | 0.5 |
aOnly changes involved in resistance are shown, and double underlining indicates that the residue is located in a gene with a mosaic structure. Additional amino acid changes, not involved in resistance, were: ParC D78A (the CipR-71 strain with no mutations that has a mosaic parC gene), R36C (3 strains), ParC K137N (27 strains), ParC N91D (the three strains with mosaic parC genes), ParE I460V (40 strains), ParE453S (1 strain), GyrA V88I (3 strains), GyrA S114G (the two strains with mosaic gyrA genes), and GyrA N150H (one of the two strains with a mosaic gyrA gene). CIP, ciprofloxacin; LVX, levofloxacin; GAT, gatifloxacin; MXF, moxifloxacin; GEMI, gemifloxacin.
1Spanish Pneumococcal Infection Study Network—general coordination: Román Pallarés; participants and centers: Ernesto García (Centro de Investigaciones Biológicas, Madrid); Julio Casal, Asuncion Fenoll, Adela G. de la Campa (Centro Nacional de Microbiología, Instituto de Salud Carlos III, Madrid); Emilio Bouza, (Hospital Gregorio Marañon, Madrid); Fernando Baquero (Hospital Ramón y Cajal, Madrid); Francisco Soriano, José Prieto (Fundación Jiménez Díaz y Hospital Clínico, Madrid); Román Pallarés, Josefina Liñares (Hospital Universitari de Bellvitge, Barcelona); Javier Garau, Javier Martínez Lacasa (Hospital Mutua de Terrassa, Barcelona); Cristina Latorre (Hospital Sant Joan de Deu, Barcelona); Emilio Pérez-Trallero, Alberto González (Hospital Donostia, San Sebastian); Juan García de Lomas (Hospital Clínico, Valencia); and Ana Fleites (Hospital Central de Asturias).