Table 1

Cats as a Risk for Transmission of Antimicrobial drug−resistant Salmonella

Filip Van Immerseel*

, Frank Pasmans*, Jeroen De Buck*, Ivan Rychlik†, Helena Hradecka†, Jean-Marc Collard‡, Christa Wildemauwe§, Marc Heyndrickx¶, Richard Ducatelle*, and Freddy Haesebrouck*

Author affiliations: *Ghent University, Merelbeke, Belgium; †Veterinary Research Institute, Brno, Czech Republic; ‡Scientific Institute of Public Health, Brussels, Belgium; §Pasteur Institute of Brussels, Brussels, Belgium; ¶Center for Agricultural Research, Melle, Belgium.

Main Article

List of primers used in the PCR reactions for detection of resistance genes

Resistance	Gene	Primer	Sequence (5′– 3′)	Size (bp)	Reference
Ampicillin	blaPSE1	PSEF	TAG CCA TAT TAT GGA GCC TC	321	AF261825
		PSER	TTA ACT TTT CCT TGC TCA GC
	bla^TEM	TEMF	GCA CGA GTG GGT TAC ATC GA	310	9
		TEMR	GGT CCT CCG ATC GTT GTC AG
	blaoxa1	oxa1F	AGC AGC GCC AGT GCA TCA	708	10
		oxa1R	ATT CGA CCC CAA GTT TCC
Chloramphenicol	floR	floRF	GCG ATA TTC ATT ACT TTG GC	425	11
		floRR	TAG GAT GAA GGT GAG GAA TG
	Cat	catF	CCT GCC ACT CAT CGC AGT	623	10
		catR	CCA CCG TTG ATA TAT CCC
Streptomycin	aadA1	aad1For	CGA CTC AAC TAT CAG AGG TA	384	AY534545
		aad1Rev	CTT TTG TCA GCA AGA TAG CC
	aadA2	aadA2F	CGG TGA CCA TCG AAA TTT CG	249	12
		aadA2R	CTA TAG CGC GGA GCG TCT CGC
	strA	strAF	CCT ATC GGT TGA TCA ATG TC	250	11
		strAR	GAA GAG TTT TAG GGT CCA CC
Tetracycline	tetA	tetAF	GCT ACA TCC TGC TTG CCT TC	210	13
		tetAR	CAT AGA TCG CCG TGA AGA GG
	tetB	tetBF	TTG GTT AGG GGC AAG TTT TG	659	13
		tetBR	GTA ATG GGC CAA TAA CAC CG
	tetC	tetCF	GCG GGA TAT CGT CCA TTC CG	207	14
		tetCR	GCG TAG AGG ATC CAC AGG ACG
	tetG	tetGF	GCT CGG TGG TAT CTC TGC TC	468	13
		tetGR	AGC AAC AGA ATC GGG AAC AC
Sulfonamide	sul1	sul1F	ATG GTG ACG GTG TTC GGC ATT CTG	841	15
		sul1R	GCT AGG CAT GAT CTA ACC CTC GG
	sul2	sul2F	AGG GGG CAG ATG TGA TCG AC	249	11
		sul2R	GCA GAT GAT TTC GCC AAT TG
Trimethoprim	dfrA1	dfrA1F	GTG AAA CTA TCA CTA ATG G	470	10
		dfrA1R	CCC TTT TGC CAG ATT TGG
	dfrA10	dfrA10F	TTA ATT ACC AGA GCA TTC GG	374	AY049746
		dfrA10R	TAC ACA TCA GCA TGA ACA GG
	dfrA12	dfrA12F	ACT CGG AAT CAG TAC GCA	463	10
		dfrA12R	GTG TAC GGA ATT ACA GCT
Kanamycin	aadD	aadD¬F	ATA TTG GAT AAA TAT GGG GAT	161	12
		aadD¬R	TCC ACC TTC CAC TCA CCG GTT
	aphA/aph(3′)¬Id	aphAaphIdF	ATG GGC GCC TAT CAC AAT TGG	257	12
		aphAaphIdR	TCG CCT CCA GCT CTT CGT AGA
	aphAI¬IAB	aphAI¬IABF	AAA CGT CTT GCT CGA GGC	461	12
		aphAI¬IABR	CAA ACC GTT ATT CAT TCG TGA
	aph(3′)¬IIa	KanAphF	GAG AAA GTA TCC ATC ATG GC	465	L19385
		KanAphR	GCT CAG AAG AAC TCG TCA AG

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Page created: April 14, 2011

Page updated: April 14, 2011

Page reviewed: April 14, 2011

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