Volume 10, Number 2—February 2004
THEME ISSUE
2004 SARS Edition
SARS Origins
Susceptibility of Pigs and Chickens to SARS Coronavirus
Hana M. Weingartl*
, John Copps*, Michael A. Drebot*, Peter Marszal*, Greg Smith*, Jason Gren*, Maya Andonova*, John Pasick*, Paul Kitching*, and Markus Czub*
, John Copps*, Michael A. Drebot*, Peter Marszal*, Greg Smith*, Jason Gren*, Maya Andonova*, John Pasick*, Paul Kitching*, and Markus Czub*
Table 1
Relative sensitivity of virus isolation and RT-PCR in tissue samples spiked with the SARS virus before homogenizationa,b
| Tissue samples | Virus isolation (PFU/100 μL) |
RT-PCR (100 μL) |
|||
|---|---|---|---|---|---|
| Vero E6 | Vero V76 | NML primers | N primers | BNI primers | |
| Virus-spiked control |
‑5.8 |
‑6 |
‑8 |
‑10 |
‑10 |
| Chicken lung |
‑5.6 |
‑5.75 |
‑7 |
‑9 |
‑9 |
| Pig lung | ‑5.5 | ‑5.7 | ‑7 | ‑9 | ‑9 |
aRT-PCR, reverse transcriptase–polymerase chain reaction; SARS, severe acute respiratory syndrome; N, nucleocapsid.
bThe highest dilution in which the virus or the RNA were detected is given in log10.
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