Volume 10, Number 5—May 2004
Research
Acute Tick-borne Rickettsiosis Caused by Rickettsia heilongjiangensis in the Russian Far East
Table 1
List of the primers used to detect rickettsial DNAa
Gene for amplification | Primer name | Primer sequence 5´-3´a | Annealing temperature |
---|---|---|---|
gltA | CS1d | ATGACTAATGGCAATAATAA | |
CS2d | ATGACCAATGAAAATAATAAT | 50°C | |
CSEndr | CTTATACTCTCTATGTACA | ||
RpCS877p | GGGGACCTGCTCACGGCGG | 54°C | |
RpCS1258n | ATTGCAAAAAGTACAGTGAACA | ||
ompA, 5´- portion | 190-70 | ATGGCGAATATTTCTCCAAAA | 53°C |
190-701 | GTTCCGTTAATGGCAGCATCT | ||
ompA, 3´- portion | 190-3588f | AACAGTGAATGTAGGAGCAG | 46°C for the first round 50°C for the nested and heminested rounds |
190-3968r | TAGCAGCTGATTTAGTAGCT | ||
190-4084f | CATCACCGATATTTCTAGC | ||
190-4338f | TTCAGGAAACGACCGTACG | ||
190-4406r | ACTATACCCTCATCGTCATT | ||
190-4859f | GCGAAATCCAAGGTACAGG | ||
190-5044r | AACTTGTAGCACCTGCCGT | ||
190-5125f | GCGGTTACTTTAGCCAAAGG | ||
190-5238r | ACTATTAAAGGCTAGGCTATT | ||
190-5768f | CACCGCTACAGGAAGCAGAT | ||
190-5831r | GTGTCGCTAGGTTTTACAAC | ||
190-6228f | CGTTGAAGTATAGCATC | ||
190-6427r | ATCTAAGCCCAGCTAGCGGT | ||
190-6808r | CACGAACTTTCACACTACC | ||
ompB |
120-M36 |
TTCTACAGCTACCATAGTAGCCA |
50°C |
120-607 | AATATCGGTGACGGTCAAGG | ||
120-807 | CCTTTTAGATTACCGCCTAA | ||
120-1378 | TAAACTTGCTGACGGTACAG | ||
120-1497 | CCTATATCGCCGGTAATT | ||
120-2113 | CGATGCTAACGTAGGTTCTT | ||
120-2339 | CTTGTTTGTTTAATGTTACGGT | ||
120-2778 | AAACAATAATCAAGGTACTGT | ||
120-2988 | CCGGCTATACCGCCTGTAGT | ||
120-3462 | CCACAGGAACTACAACCATT | ||
120-3599 | TACTTCCGGTTACAGCAAAGT | ||
120-4232 | GGTTTCTCATTCTCTCTATATGG | ||
120-4346 | CGAAGAAGTAACGCTGACTT | ||
120-4879 | TTAGAAGTTTACACGGACTTTT |
aDifferences between primers CS1d and CS2d are indicated by bold letters.
Page created: July 05, 2012
Page updated: July 05, 2012
Page reviewed: July 05, 2012
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