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Volume 11, Number 10—October 2005
Research

Botulinum Neurotoxin Detection and Differentiation by Mass Spectrometry

John R. Barr*Comments to Author , Hercules Moura*, Anne E. Boyer*, Adrian R. Woolfitt*, Suzanne R. Kalb*, Antonis Pavlopoulos*, Lisa G. McWilliams†, Jurgen G. Schmidt‡, Rodolfo A. Martinez‡, and David C. Ashley*
Author affiliations: *Centers for Disease Control and Prevention, Atlanta, Georgia, USA; †Battelle Memorial Institute, Atlanta, Georgia, USA; ‡Los Alamos National Laboratory, Los Alamos, New Mexico, USA

Main Article

Figure A1

Matrix-assisted laser desorption time-of-flight mass spectrometry (MALDI-TOF-MS) spectrum of botulinum neurotoxin (BoNT) reactions. The top panel shows the MALDI-TOF-MS spectrum of the mixture of substrate peptides with no toxin present. The second panel shows this same mixture of substrate peptides and BoNT-A. The third, fourth and fifth panels show the mixture of substrate peptides with BoNT-B, -E, and –F, respectively. The insert mass spectrum for BoNT-E distinguishes the BoNT-dependent N-ter

Figure A1. Matrix-assisted laser desorption time-of-flight mass spectrometry (MALDI-TOF-MS) spectrum of botulinum neurotoxin (BoNT) reactions. The top panel shows the MALDI-TOF-MS spectrum of the mixture of substrate peptides with no toxin present. The second panel shows this same mixture of substrate peptides and BoNT-A. The third, fourth and fifth panels show the mixture of substrate peptides with BoNT-B, -E, and –F, respectively. The insert mass spectrum for BoNT-E distinguishes the BoNT-dependent N-terminal product from the BoNT-A substrate peptide.

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Main Article

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