Volume 11, Number 10—October 2005
Dispatch
Detecting Biological Warfare Agents
Table A1
Sequence information of probes and primer pairs* (Download PDF [47 KB, 1 page])
Organism | Target gene | Accession no. | Probe sequences and primer sequences (5’-3’) | PCR product (bp) | Name |
---|---|---|---|---|---|
Bacillus anthracis | capA | M24150 | P: 5´-CCGAGTCCTAGACAGGAAGCCTTAGCAAAAGCAATGGTTGATGCAGGGGC-3´ F: 5´-GGGGGGAAGAATACGATAAT-3´; R: 5´-GTTCTTGTCCATCCTTGGTC-3’ | 188 | BA1 |
P: 5´-ACGCACTGGGGGGAAGAATACGATAATAAACCGAGTCCTAGACAGGAAGC-3´ F: 5´-TAGTAAATACGCACTGGGTT-3´; R: 5’-CATCCTTGGTCAAACACAAA-3´ | 194 | BA2 | |||
pagA | M22589 | P: 5´-GGAAGAGTGAGGGTGGATACAGGCTCGAACTGGAGTGAAGTGTTACCGCA-3´ F: 5´-AAAATGGAAGAGTGAGGGTG-3´; R: 5´-CCGCCTTTCTACCAGATTTA-3´ | 120 | BA3 | |
P: 5´-TGTATCACCAGAGGCAAGACACCCCCTTGTGGCAGCTTATCCGATTGTAC-3´ F: 5´-TGTATCACCAGAGGCAAGAC-3´; R: 5´-TTCTGTGTGGATTGATCCTC-3´ | 106 | BA4 | |||
SASP-B | AF359938 | P: 5´-GAAGCTAAGAAAGCGCAAGCTTCTGGTGCTAGATTCAAAGCACAAATGC-3´ F: 5´-GTAAAACAAGCAAACGCACA-3´; R: 5´-GATTGTGATTGTTTTGCAGC-3´ | 162 | BA5 | |
P: 5´-CAAGCAGTAAAACAAGCAAACGCACAATCAGAAGCTAAGAAAGCGCAAGC-3´ F: 5´-TTTGCGACTGAAACAAATGT-3´; R: 5´-ATGCACGTCTGTTTCAGTTG-3´ | 142 | BA6 | |||
Brucella melitensis | Sequence between BMEI1658 and BMEI1659† | AE009601 | P: 5´-AGGTGTCATTAGGCTTGCTTGTGGACGAGAGAACGGTGGACCAAGGGAGA-3´ F: 5´-TCCGAGGGTAAACACCTAAG-3´; R: 5´-ACTCACGGACAGCATTAGGT-3´ | 181 | BM1 |
P: 5´-CTCTCACGGTCCCCATAATGGAGAGTGATCCGAGGGTAAACACCTAAGGG-3´ F: 5´-TCTCTCACGGTCCCCATAAT-3´; R: 5´-AGCAAGCCTAATGACACCTT-3´ | 147 | BM2 | |||
Clostridium botulinum | Random chromosomal DNA fragment‡ | NA | P: 5´- CATCTTCACGGAGGTGAACAAGCCTCCATGTTCGACGGTAACCCAGAAGC-3´ F: 5´-AGATCAGAAGGATGTACCGC-3´; R: 5´-ACTCTTGGACATTGGGGTAA-3´ | 173 | CB1 |
P: 5´-CCACCGGGAGTGCCGGAAGATCAGAAGGATGTACCGCTAGTAACTCATCT-3´ F: 5´-AATAATACGCCTATGACGCC-3´; R: 5´-TAAACCTTTTGCTGTCCACC-3´ | 163 | CB2 | |||
Yersinia pestis | Plasminogen activator | M27820 | P: 5´-GGACGTCTCTGGCTTCCGGGTCAGGTAATATGGATGACTACGACTGGATG-3´ F: 5´-CAAAAATGTCGCTATCCTGA-3´; R: 5´-GGTCATATTCATTGGCATGA-3´ | 201 | YP1 |
P: 5´-TCCATACTCATTTCTGACCCTGAATGCCAGGGGGTGGACGTCTCTGGCTT-3´ F: 5´-ACGCAGAAACAGGAAGAAAG-3´; R: 5´-CATCCAGTCGTAGTCATCCA-3´ | 168 | YP2 | |||
Bacillus thuringiensis kurstaki | cry1A | AY225453 | P: 5´-TGGTCAGGGCATCAAATAATGGCTTCTCCTGTCGGTTTTTCGGGGCCAGA-3’ F: 5´-TATTATTGGTCAGGGCATCA-3´; R: 5´-GTTCTATACACGCCCTGACC-3´ | 144 | BTK1 |
P: 5´-GGTAGTTTTCGAGGCTCGGCTCAGGGCATAGAAAGAAGTATTAGGAGTCC-3´ F: 5´-TGATGGTAGTTTTCGAGGCT-3´; R: 5´-CGACAGGAGAAGCCATTATT-3´ | 153 | BTK2 | |||
Vaccinia virus | Thymidine kinase | U94848 | P: 5´-CGTATGGCAAACGAAGGAAAAATAGTTATAGTAGCCGCACTCGATGGGAC-3´ F: 5´-ATTCTGTGAGCGTATGGCAA-3´; R: 5´-TCTATCTCGGTTTCCTCACC-3´ | 199 | VA1 |
P: 5´-GGCGGACATATTCAGTTGATAATCGGCCCCATGTTTTCAGGTAAAAGTAC-3´ F: 5´-GGCGGACATATTCAGTTGAT-3´; R: 5´-GCTTCCAATGCTTCAAAATT-3´ | 183 | VA2 | |||
Francisella tularensis | fopA | AF097542 | P: 5´-GGTTCAGCTACAGCATCTATCGCTGCAGGTTCAGATAATATCGATACATC-3´ F: 5´-TTAGGTTCAGCTACAGCATC-3´; R: 5´-TGTCCTTGTTAGTCAAAGCG-3´ | 155 | FT1 |
P: 5´-CATCAGCAAACACTAATTCAGCTACTACACAAAGCAGTGGTTTTGCAGCT-3´ F: 5´-TCAGCTACAGCATCTATCGC-3´; R: 5’-TGTCCTTGTTAGTCAAAGCG-3’ | 149 | FT2 |
*P, probe; F, forward primer; R, reverse primer; NA, not applicable. All sequences were purchased from Integrated DNA Technologies, Inc. (Coralville, IA, USA). Probe sequences were modified with an amine group and C6 linker at the 5´ position, and these were used to immobilize the probe on microspheres. Synthetic complementary targets of probes and reverse primers were modified with Cy3 at 5´ position as a reporter for array hybridization.
†Sequences are from the noncoding region between 2 open reading frames, BMEI1658 and BMEI1659.
‡Sequences were obtained by sequencing by the collaborator and are not available from GenBank.