Volume 11, Number 11—November 2005
Dispatch
Anaplasma phagocytophilum–infected Ticks, Japan
Table
Polymerase chain reaction (PCR) detection of Anaplasma phagocytophilum p44/msp2 paralogs from Ixodes ticks or spleens of mice experimentally infected with tick tissues
Collection site, year* | Whole tissue† |
Salivary gland† |
Experimental infection with ticks‡ |
Total | ||
---|---|---|---|---|---|---|
Female | Male | Female | Female | Male | ||
I. persulcatus | ||||||
Yamanashi, 2004 | ||||||
TN | 0/2 | 0/4 | 0/6 | |||
UM | 2/16 | 2/17 | 4/33 | |||
Nagano, 2004 | ||||||
UG | 0/3 | 0/0 | 0/3 | |||
Shizuoka, 2004 | ||||||
TK | 6/9 | 6/9 | ||||
MZ | 1/8 | 1/8 | ||||
Shizuoka, 2003 | ||||||
TK | 0/14 (2) | 0/10 (1) | 0/24 (3) | |||
MZ | 0/22 (2) | 0/9 (1) | 0/31 (3) | |||
Total |
2/21 |
2/21 |
7/17 |
0/36 (4) |
0/19 (2) |
11/114 (6) |
I. ovatus | ||||||
Yamanashi, 2004 | ||||||
TN | 0/8 | 0/8 | 0/16 | |||
UM | 0/9 | 0/3 | 0/12 | |||
Nagano, 2004 | ||||||
UG | 0/1 | 0/2 | 0/3 | |||
Shizuoka, 2004 | ||||||
TK | 9/17 | 9/17 | ||||
MZ | 0/16 | 0/16 | ||||
Shizuoka, 2003 | ||||||
TK | 0/32 (3) | 1/16 (2) | 1/48 (5) | |||
MZ | 0/26 (2) | 0/21 (2) | 0/47 (4) | |||
Total | 0/18 | 0/13 | 9/33 | 0/58 (5) | 1/37 (4) | 10/159 (9) |
*TN, Tennyosan; UM, Utsukushinomori; UG, Utsukushigahara; TK, Takabachi; MZ, Mizugazuka.
†No. positive/no. examined. One hundred twenty-three ticks were dissected (whole tissues from 73 ticks and salivary glands from 50) were individually examined by PCR.
‡No. of positive mouse spleens/no. of ticks examined (no. of mice used). Six to 15 ticks were pooled and homogenized (55 I. persulcatus and 95 I. ovatus), and intraperitoneally injected into ddY male mice.