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Volume 11, Number 11—November 2005
Dispatch

Anaplasma phagocytophilum–infected Ticks, Japan

Norio Ohashi*†Comments to Author , Megumi Inayoshi*‡, Kayoko Kitamura*, Fumihiko Kawamori*‡, Daizoh Kawaguchi*, Yuusaku Nishimura*, Hirotaka Naitou*, Midori Hiroi*‡, and Toshiyuki Masuzawa*†
Author affiliations: *University of Shizuoka, Shizuoka, Japan; †Center of Excellence Program in the 21st Century, Shizuoka, Japan; ‡Shizuoka Institute of Environment and Hygiene, Shizuoka, Japan

Main Article

Table

Polymerase chain reaction (PCR) detection of Anaplasma phagocytophilum p44/msp2 paralogs from Ixodes ticks or spleens of mice experimentally infected with tick tissues

Collection site, year* Whole tissue†
 Salivary gland†
 Experimental infection with ticks‡
 Total
Female Male Female Female Male
I. persulcatus
Yamanashi, 2004
TN 0/2 0/4 0/6
UM 2/16 2/17 4/33
Nagano, 2004
UG 0/3 0/0 0/3
Shizuoka, 2004
TK 6/9 6/9
MZ 1/8 1/8
Shizuoka, 2003
TK 0/14 (2) 0/10 (1) 0/24 (3)
MZ 0/22 (2) 0/9 (1) 0/31 (3)
Total
 2/21
 2/21
 7/17
 0/36 (4)
 0/19 (2)
 11/114 (6)
I. ovatus
Yamanashi, 2004
TN 0/8 0/8 0/16
UM 0/9 0/3 0/12
Nagano, 2004
UG 0/1 0/2 0/3
Shizuoka, 2004
TK 9/17 9/17
MZ 0/16 0/16
Shizuoka, 2003
TK 0/32 (3) 1/16 (2) 1/48 (5)
MZ 0/26 (2) 0/21 (2) 0/47 (4)
Total 0/18 0/13 9/33 0/58 (5) 1/37 (4) 10/159 (9)

*TN, Tennyosan; UM, Utsukushinomori; UG, Utsukushigahara; TK, Takabachi; MZ, Mizugazuka.
†No. positive/no. examined. One hundred twenty-three ticks were dissected (whole tissues from 73 ticks and salivary glands from 50) were individually examined by PCR.
‡No. of positive mouse spleens/no. of ticks examined (no. of mice used). Six to 15 ticks were pooled and homogenized (55 I. persulcatus and 95 I. ovatus), and intraperitoneally injected into ddY male mice.

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Page created: February 17, 2012
Page updated: February 17, 2012
Page reviewed: February 17, 2012
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