Skip directly to site content Skip directly to page options Skip directly to A-Z link Skip directly to A-Z link Skip directly to A-Z link
Volume 11, Number 11—November 2005
Dispatch

Anaplasma phagocytophilum–infected Ticks, Japan

Norio Ohashi*†Comments to Author , Megumi Inayoshi*‡, Kayoko Kitamura*, Fumihiko Kawamori*‡, Daizoh Kawaguchi*, Yuusaku Nishimura*, Hirotaka Naitou*, Midori Hiroi*‡, and Toshiyuki Masuzawa*†
Author affiliations: *University of Shizuoka, Shizuoka, Japan; †Center of Excellence Program in the 21st Century, Shizuoka, Japan; ‡Shizuoka Institute of Environment and Hygiene, Shizuoka, Japan

Main Article

Table

Polymerase chain reaction (PCR) detection of Anaplasma phagocytophilum p44/msp2 paralogs from Ixodes ticks or spleens of mice experimentally infected with tick tissues

Collection site, year* Whole tissue†
Salivary gland†
Experimental infection with ticks‡
Total
Female Male Female Female Male
I. persulcatus
Yamanashi, 2004
TN 0/2 0/4 0/6
UM 2/16 2/17 4/33
Nagano, 2004
UG 0/3 0/0 0/3
Shizuoka, 2004
TK 6/9 6/9
MZ 1/8 1/8
Shizuoka, 2003
TK 0/14 (2) 0/10 (1) 0/24 (3)
MZ 0/22 (2) 0/9 (1) 0/31 (3)
Total
2/21
2/21
7/17
0/36 (4)
0/19 (2)
11/114 (6)
I. ovatus
Yamanashi, 2004
TN 0/8 0/8 0/16
UM 0/9 0/3 0/12
Nagano, 2004
UG 0/1 0/2 0/3
Shizuoka, 2004
TK 9/17 9/17
MZ 0/16 0/16
Shizuoka, 2003
TK 0/32 (3) 1/16 (2) 1/48 (5)
MZ 0/26 (2) 0/21 (2) 0/47 (4)
Total 0/18 0/13 9/33 0/58 (5) 1/37 (4) 10/159 (9)

*TN, Tennyosan; UM, Utsukushinomori; UG, Utsukushigahara; TK, Takabachi; MZ, Mizugazuka.
†No. positive/no. examined. One hundred twenty-three ticks were dissected (whole tissues from 73 ticks and salivary glands from 50) were individually examined by PCR.
‡No. of positive mouse spleens/no. of ticks examined (no. of mice used). Six to 15 ticks were pooled and homogenized (55 I. persulcatus and 95 I. ovatus), and intraperitoneally injected into ddY male mice.

Main Article

Page created: February 17, 2012
Page updated: February 17, 2012
Page reviewed: February 17, 2012
The conclusions, findings, and opinions expressed by authors contributing to this journal do not necessarily reflect the official position of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.
file_external