Figure A4. Alignment of Passatempo virus (PSTV) hemagluttinin antigen (HA) and vaccinia grown factor (VGF) amino acid inferred sequences with other orthopoxvirus sequences. 1, HA sequence of PSTV (DQ070848) was compared to the region corresponding to amino acids 6 to 309 of vaccinia virus (VACV-WR, AY243312) HA sequence and to HA sequences of Araçatuba virus (ARAV, AY523994); Cantagalo virus (CTGV, AF229247); vaccinia virus IOC (VACV-IOC, AF229248); vaccinia virus Lister (VAVC-LST, AF375124) and cowpox virus Brighton Red (BR) (CPXV-BR, AF482758). 2– The VGF sequence of PSTV (DQ085462) was compared to the region corresponding to amino acids 19 to 135 of vaccinia virus WR (VACV-WR, AY243312) and to the same region of Araçatuba virus (ARAV, AF503170), vaccinia virus Lister (VACV-LST, AY678276); and cowpox virus BR (CPXV-BR, AF482758). The sequences were obtained from GenBank and aligned by using the default parameters of CLUSTAL W and hand-edited to enhance biologically significant regions. The amino acid positions are shown according to the VACV-WR HA and VGF protein sequences. (.) indicates identity of amino acids, (-) indicates deletions, and (*) indicates the PSTV amino acid substitutions. The box shows the conserved deletion observed in the HA sequence of the Brazilian VACVs. Amino acids substitution found amongst PSTV and other Brazilian VACVs are shown in gray boxes.