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Volume 11, Number 12—December 2005
Dispatch

Passatempo Virus, a Vaccinia Virus Strain, Brazil

Juliana A. Leite*, Betânia P. Drumond*, Giliane S. Trindade*, Zélia I.P. Lobato*, Flávio G. da Fonseca†, João R. dos Santos*, Marieta C. Madureira‡, Maria I.M.C. Guedes*, Jaqueline M.S. Ferreira*, Cláudio A. Bonjardim, Paulo C.P. Ferreira*, and Erna G. Kroon*Comments to Author 
Author affiliations: *Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil; †Centro de Pesquisas René Rachou–Fundação Instituto Oswaldo Cruz, Belo Horizonte, Minas Gerais, Brazil; ‡Instituto Mineiro de Agropecuária, Belo Horizonte, Minas Gerais, Brazil

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Figure A3

Restriction fragment length polymorphism analysis of the ati gene of viral isolates. ati fragments were amplified by polymerase chain reaction by using primers based on the cowpox virus ati gene nucleotide sequence, followed by XbaI digestion. The amplified DNA was fractionated by electrophoresis on a 1% agarose gel and stained with ethidium bromide. Lanes: 1, vaccinia virus WR; 2, isolate-cow1; 3, isolate-cow2; 4, isolate-cow3; 5, isolate-cow4; 6, isolate-cow5; 7, isolate-calf; 8, cowpox virus

Figure A3. Restriction fragment length polymorphism analysis of the ati gene of viral isolates. ati fragments were amplified by polymerase chain reaction by using primers based on the cowpox virus ati gene nucleotide sequence, followed by XbaI digestion. The amplified DNA was fractionated by electrophoresis on a 1% agarose gel and stained with ethidium bromide. Lanes: 1, vaccinia virus WR; 2, isolate-cow1; 3, isolate-cow2; 4, isolate-cow3; 5, isolate-cow4; 6, isolate-cow5; 7, isolate-calf; 8, cowpox virus BR.

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