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Volume 11, Number 2—February 2005
Dispatch

Diagnostic System for Rapid and Sensitive Differential Detection of Pathogens

Thomas Briese*1, Gustavo Palacios*1, Mark Kokoris†1, Omar Jabado*, Zhiqiang Liu*, Neil Renwick*, Vishal Kapoor*, Inmaculada Casas‡, Francisco Pozo‡, Ron Limberger§, Pilar Perez-Brena‡, Jingyue Ju*, and W. Ian Lipkin*Comments to Author 
Author affiliations: *Columbia University, New York, New York, USA; †Qiagen Inc., Valencia, California, USA; ‡Instituto de Salud Carlos III, Majadahonda, Madrid, Spain; §New York State Department of Health, Albany, New York, USA

Main Article

Table 1

Sensitivity of pathogen detection by Mass Tag polymerase chain reaction determined by using plasmid and synthetic RNA standards*

Pathogen or protein Detection threshold (DNA copies/RNA copies)
Influenza A matrix 100/1,000
Influenza A N1 100/NA
Influenza A N2 100/NA
Influenza A H1 100/NA
Influenza A H2 100/NA
Influenza A H3 100/NA
Influenza A H5 100/NA
Influenza B H 500/1,000
RSV group A 100/1,000
RSV group B 100/500
Metapneumovirus 100/1,000
CoV-SARS 100/500
CoV-OC43 100/500
CoV-229E 100/500
HPIV-1 100/1,000
HPIV-2 100/1,000
HPIV-3 100/500
Chlamydia pneumoniae 100/NA
Mycoplasma pneumoniae 100/NA
Legionella pneumophila 100/NA
Enterovirus (genus) 500/1,000
Adenovirus (genus) 5,000/NA

*NA, not assessed; RSV, respiratory syncytial virus; CoV, coronavirus; SARS, severe acute respiratory syndrome; HPIV, human parainfluenza virus.

Main Article

1These authors contributed equally to this study.

Page created: April 17, 2012
Page updated: April 17, 2012
Page reviewed: April 17, 2012
The conclusions, findings, and opinions expressed by authors contributing to this journal do not necessarily reflect the official position of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.
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