Volume 11, Number 2—February 2005
Dispatch
Molecular Evidence of Pneumocystis Transmission in Pediatric Transplant Unit
Britta Höcker*
, Constanze Wendt†, Aimable Nahimana‡, Burkhard Tönshoff*, and Philippe M. Hauser‡
, Constanze Wendt†, Aimable Nahimana‡, Burkhard Tönshoff*, and Philippe M. Hauser‡
Table
Pneumocystis jirovecii genotyping by PCR-SSCP of 4 genomic regions*
| Patient | Date | SSCP pattern |
||||
|---|---|---|---|---|---|---|
| ITS1 | 26S | mt26 | β-tub | P. jirovecii type | ||
| Index case-patient | 3/27/02 | A | A, B | A, B,C | A, B | >2 types (nonidentifiable) |
| 1 | 7/26/02 | A | A, B | A | A | 1, 2 |
| 2 | 10/30/02 | A | A, B | A | A | 1, 2 |
| 3 | 12/3/02 | A | A, B | A | A | 1, 2 |
| 4 | 2/7/03 | A, B | A, B, C | B, C | B, C | >2 types (nonidentifiable) |
| 5 | 2/15/03 | A | A, B | C | A | 6, 44 |
| 6 | 2/18/03 | A, B | A, B | A | C | 45, 46 |
*Bold letters signify the most abundant simple pattern with the complex one, as shown by silver staining. Bold numbers signify the most abundant P. jirovecii type. PCR, polymerase chain reaction; SSCP, multitarget single-strand conformation polymorphism.
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