Volume 11, Number 8—August 2005
Dispatch
Influenza A H5N1 Detection
Table 2
Detection sensitivity of polymerase chain reaction and rapid antigen test for H5N1*
Test |
Dilution† | |||||
---|---|---|---|---|---|---|
103 |
104 |
105 |
106 |
107 |
108 |
|
Rapid antigen test‡ | + | + | – | – | – | – |
Conventional nested RT-PCR | + | + | + | + | – | – |
Real-time RT-PCR | + | + | + | + | + | – |
*RT-PCR, reverse transcription–polymerase chain reaction.
†Serial 10-fold dilution with a concentration of 108 50% tissue culture infective doses/mL of rhabdomyosarcoma cell culture fluid.
‡Binax NOW; +, positive; –, negative.