Volume 11, Number 8—August 2005
Dispatch
Influenza A H5N1 Detection
Enders K.O. Ng*, Peter K.C. Cheng*, Antia Y.Y. Ng*, T.L. Hoang†, and Wilina W.L. Lim*
Table 2
Detection sensitivity of polymerase chain reaction and rapid antigen test for H5N1*
| Test |
Dilution† | |||||
|---|---|---|---|---|---|---|
| 103 |
104 |
105 |
106 |
107 |
108 |
|
| Rapid antigen test‡ | + | + | – | – | – | – |
| Conventional nested RT-PCR | + | + | + | + | – | – |
| Real-time RT-PCR | + | + | + | + | + | – |
*RT-PCR, reverse transcription–polymerase chain reaction.
†Serial 10-fold dilution with a concentration of 108 50% tissue culture infective doses/mL of rhabdomyosarcoma cell culture fluid.
‡Binax NOW; +, positive; –, negative.
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