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Volume 12, Number 3—March 2006

Host Feeding Patterns of Culex Mosquitoes and West Nile Virus Transmission, Northeastern United States

Goudarz Molaei*Comments to Author , Theodore G. Andreadis*, Philip M. Armstrong*, John F. Anderson*, and Charles R. Vossbrinck*
Author affiliations: *The Connecticut Agricultural Experiment Station, New Haven, Connecticut, USA

Main Article

Table 2

Sequences of primers, length of amplification products, and thermal cycling conditions used in polymerase chain reactions for blood-meal analysis

Primer name Sequence Product (bp) Cycling condition
Denaturation Annealing Extension No. cycles
Avian a GAC TGT GAC AAA ATC CCN TTC CA (f)* 508 94°C (30 s) 60°C (50 s) 72°C (40 s) 36
Avian b CCC TCA GAA TGA TAT TTG TCC TCA (f)† 515 95°C (1 min) 58°C (1 min) 72°C (1 min) 35
Mammalian a CGA AGC TTG ATA TGA AAA ACC ATC GTT G (f) 772 94°C (30 s) 55°C (45 s) 72°C (1.5 min) 36
Mammalian b GCG TAC GCA ATC TTA CGA TCA A (f) 195 95°C (1 min) 54°C (1 min) 72°C (1 min) 32
Mammalian c CCA TCC AAC ATC TCA GCA TGA TGA AA (f) 395 95°C (1 min) 55°C (1 min) 72°C (1 min) 32

*Reference (39)
†Reference (40)

Main Article

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