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Volume 12, Number 4—April 2006
Dispatch

MassTag Polymerase Chain Reaction for Differential Diagnosis of Viral Hemorrhagic Fevers

Gustavo Palacios*1, Thomas Briese*1Comments to Author , Vishal Kapoor*, Omar Jabado*, Zhiqiang Liu*, Marietjie Venter†, Junhui Zhai*, Neil Renwick*, Allen Grolla‡, Thomas W. Geisbert§, Christian Drosten¶, Jonathan S. Towner#, Jingyue Ju*, Janusz Paweska**, Stuart T. Nichol#, Robert Swanepoel**, Heinz Feldmann‡††, Peter B. Jahrling‡‡, and W. Ian Lipkin*
Author affiliations: *Columbia University, New York, New York, USA; †University of Pretoria and National Health Laboratory Services, Pretoria, South Africa; ‡Public Health Agency of Canada, Winnipeg, Manitoba, Canada; §United States Army Medical Research Institute of Infectious Diseases, Fort Detrick, Frederick, Maryland, USA; ¶Bernhard-Nocht-Institute of Tropical Medicine, Hamburg, Germany; #Centers for Disease Control and Prevention, Atlanta, Georgia, USA; **National Institute for Communicable Diseases, Sandringham, South Africa; ††University of Manitoba, Winnipeg, Manitoba, Canada; ‡‡National Institutes of Allergy and Infectious Diseases Integrated Research Facility, Fort Detrick, Frederick, Maryland, USA

Main Article

Table 1

Greene MassTag panel VHF version 1.0*

Target MassTag Name Sequence Gene
ZEBOV 718 (fwd) EboZA-U234 AACACCGGGTCTTAATTCTTATATCAA L
646 (rev) EboZA-L319 GGTGGTAAAATTCCCATAGTAGTTCTTT
SEBOV 503 (fwd) EboSU-U416 CGAGCCTAACGTTTTGGGC L
630 (rev) EboSU-L489 GCTCCAGGAATTGTTCGGGTA
MARV 654 (fwd) MARV-U12816C CCCTCCATATCTTAGACAACATATTGTG L
395 (rev) MARV-L12994 CCCAACACTCCTGGTTCACAGC
LASV† 558 (fwd) Las4-U92 ACTGCATTYTCATACTTYCTRGAATC NP
686 (rev) Las4-L257 CCRGGYTTGACCAGTGCTGT
RVFV 658 (fwd) RVF-U578 GGATTGACCTGTGCCTGTTGC N
495 (rev) RVF-L660 GCATTAGAAATGTCCTCTTTTGCTGC
CCHFV 499 (fwd) CCHV-U4 AGAAACACGTGCCGCTTACGCCCA N
710 (rev) CCHV-L120 CCATTTCCYTTYTTRAACTCYTCAAACCA
HNTV 479 (fwd) HAN-U179 AYACAGCAGCAGTTAGCCTCCT N
702 (rev) HAN-L245 GCT GCC GTA RGT AGT CCC TGTT
SEOV 455 (fwd) SEO-U243 CAGGATTGCAGCAGGGAAGA N
602 (rev) SEOUL-L309 ATGATCACCAGGYTCTACCCC
YFV 467 (fwd) YF-U186 GCTGGGAGCGCGGTATC NS5
670 (rev) YF-L249 GGAAGCCCAATGGTCCTCAT
KFDV 483 (fwd) KYF-U170 TGGAAGCCTGGCTGAAAGAG NS5
614 (rev) KYF-L233 TCATCCCCACTGACCAGCAT

*ZEBOV, Ebola Zaire virus; SEBOV, Ebola Sudan virus; MARV, Marburg virus; LASV, Lassa virus; RVFV, Rift Valley fever virus; CCHV, Crimean-Congo hemorrhagic fever virus; HNTV, Hantaan virus; SEOV, Seoul virus; YFV, yellow fever virus; KFDV, Kyasanur Forest disease virus; fwd, forward; rev, reverse.
†Primers were designed on Lassa lineage IV sequences (15) and the recently identified outlier sequence Lassa AV (AF256121).

Main Article

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1These authors contributed equally to this article.

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