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Volume 12, Number 4—April 2006
Dispatch

MassTag Polymerase Chain Reaction for Differential Diagnosis of Viral Hemorrhagic Fevers

Gustavo Palacios*1, Thomas Briese*1Comments to Author , Vishal Kapoor*, Omar Jabado*, Zhiqiang Liu*, Marietjie Venter†, Junhui Zhai*, Neil Renwick*, Allen Grolla‡, Thomas W. Geisbert§, Christian Drosten¶, Jonathan S. Towner#, Jingyue Ju*, Janusz Paweska**, Stuart T. Nichol#, Robert Swanepoel**, Heinz Feldmann‡††, Peter B. Jahrling‡‡, and W. Ian Lipkin*
Author affiliations: *Columbia University, New York, New York, USA; †University of Pretoria and National Health Laboratory Services, Pretoria, South Africa; ‡Public Health Agency of Canada, Winnipeg, Manitoba, Canada; §United States Army Medical Research Institute of Infectious Diseases, Fort Detrick, Frederick, Maryland, USA; ¶Bernhard-Nocht-Institute of Tropical Medicine, Hamburg, Germany; #Centers for Disease Control and Prevention, Atlanta, Georgia, USA; **National Institute for Communicable Diseases, Sandringham, South Africa; ††University of Manitoba, Winnipeg, Manitoba, Canada; ‡‡National Institutes of Allergy and Infectious Diseases Integrated Research Facility, Fort Detrick, Frederick, Maryland, USA

Main Article

Table 1

Greene MassTag panel VHF version 1.0*

Target MassTag Name Sequence Gene
ZEBOV 718 (fwd) EboZA-U234 AACACCGGGTCTTAATTCTTATATCAA L
646 (rev) EboZA-L319 GGTGGTAAAATTCCCATAGTAGTTCTTT
SEBOV 503 (fwd) EboSU-U416 CGAGCCTAACGTTTTGGGC L
630 (rev) EboSU-L489 GCTCCAGGAATTGTTCGGGTA
MARV 654 (fwd) MARV-U12816C CCCTCCATATCTTAGACAACATATTGTG L
395 (rev) MARV-L12994 CCCAACACTCCTGGTTCACAGC
LASV† 558 (fwd) Las4-U92 ACTGCATTYTCATACTTYCTRGAATC NP
686 (rev) Las4-L257 CCRGGYTTGACCAGTGCTGT
RVFV 658 (fwd) RVF-U578 GGATTGACCTGTGCCTGTTGC N
495 (rev) RVF-L660 GCATTAGAAATGTCCTCTTTTGCTGC
CCHFV 499 (fwd) CCHV-U4 AGAAACACGTGCCGCTTACGCCCA N
710 (rev) CCHV-L120 CCATTTCCYTTYTTRAACTCYTCAAACCA
HNTV 479 (fwd) HAN-U179 AYACAGCAGCAGTTAGCCTCCT N
702 (rev) HAN-L245 GCT GCC GTA RGT AGT CCC TGTT
SEOV 455 (fwd) SEO-U243 CAGGATTGCAGCAGGGAAGA N
602 (rev) SEOUL-L309 ATGATCACCAGGYTCTACCCC
YFV 467 (fwd) YF-U186 GCTGGGAGCGCGGTATC NS5
670 (rev) YF-L249 GGAAGCCCAATGGTCCTCAT
KFDV 483 (fwd) KYF-U170 TGGAAGCCTGGCTGAAAGAG NS5
614 (rev) KYF-L233 TCATCCCCACTGACCAGCAT

*ZEBOV, Ebola Zaire virus; SEBOV, Ebola Sudan virus; MARV, Marburg virus; LASV, Lassa virus; RVFV, Rift Valley fever virus; CCHV, Crimean-Congo hemorrhagic fever virus; HNTV, Hantaan virus; SEOV, Seoul virus; YFV, yellow fever virus; KFDV, Kyasanur Forest disease virus; fwd, forward; rev, reverse.
†Primers were designed on Lassa lineage IV sequences (15) and the recently identified outlier sequence Lassa AV (AF256121).

Main Article

References
  1. Peters  CJ, Zaki  SR. Role of the endothelium in viral hemorrhagic fevers. Crit Care Med. 2002;30(Suppl):S268–73. DOIPubMedGoogle Scholar
  2. Geisbert  TW, Jahrling  PB. Exotic emerging viral diseases: progress and challenges. Nat Med. 2004;10(Suppl):S110–21. DOIPubMedGoogle Scholar
  3. Pugachev  KV, Guirakhoo  F, Monath  TP. New developments in flavivirus vaccines with special attention to yellow fever. Curr Opin Infect Dis. 2005;18:387–94. DOIPubMedGoogle Scholar
  4. Pittman  PR, Liu  CT, Cannon  TL, Makuch  RS, Mangiafico  JA, Gibbs  PH, Immunogenicity of an inactivated Rift Valley fever vaccine in humans: a 12-year experience. Vaccine. 1999;18:181–9. DOIPubMedGoogle Scholar
  5. Enria  DA, Barrera Oro  JG. Junin virus vaccines. Curr Top Microbiol Immunol. 2002;263:239–61. DOIPubMedGoogle Scholar
  6. Hooper  JW, Li  D. Vaccines against hantaviruses. Curr Top Microbiol Immunol. 2001;256:171–91. DOIPubMedGoogle Scholar
  7. Dandawate  CN, Desai  GB, Achar  TR, Banerjee  K. Field evaluation of formalin inactivated Kyasanur forest disease virus tissue culture vaccine in three districts of Karnataka state. Indian J Med Res. 1994;99:152–8.PubMedGoogle Scholar
  8. Enria  DA, Maiztegui  JI. Antiviral treatment of Argentine hemorrhagic fever. Antiviral Res. 1994;23:23–31. DOIPubMedGoogle Scholar
  9. McCormick  JB, King  IJ, Webb  PA, Scribner  CL, Craven  RB, Johnson  KM, Lassa fever. Effective therapy with ribavirin. N Engl J Med. 1986;314:20–6. DOIPubMedGoogle Scholar
  10. Ozkurt  Z, Kiki  I, Erol  S, Erdem  F, Yilmaz  N, Parlak  M, Crimean-Congo hemorrhagic fever in eastern Turkey: clinical features, risk factors and efficacy of ribavirin therapy. J Infect. Epub 2005 Jun 13.PubMedGoogle Scholar
  11. Huggins  JW, Hsiang  CM, Cosgriff  TM, Guang  MY, Smith  JI, Wu  ZO, Prospective, double-blind, concurrent, placebo-controlled clinical trial of intravenous ribavirin therapy of hemorrhagic fever with renal syndrome. J Infect Dis. 1991;164:1119–27. DOIPubMedGoogle Scholar
  12. Borio  L, Inglesby  T, Peters  CJ, Schmaljohn  AL, Hughes  JM, Jahrling  PB, Hemorrhagic fever viruses as biological weapons: medical and public health management. JAMA. 2002;287:2391–405. DOIPubMedGoogle Scholar
  13. Drosten  C, Kummerer  BM, Schmitz  H, Gunther  S. Molecular diagnostics of viral hemorrhagic fevers. Antiviral Res. 2003;57:61–87. DOIPubMedGoogle Scholar
  14. Briese  T, Palacios  G, Kokoris  M, Jabado  O, Liu  Z, Renwick  N, Diagnostic system for rapid and sensitive differential detection of pathogens. Emerg Infect Dis. 2005;11:310–3.PubMedGoogle Scholar
  15. Bowen  MD, Rollin  PE, Ksiazek  TG, Hustad  HL, Bausch  DG, Demby  AH, Genetic diversity among Lassa virus strains. J Virol. 2000;74:6992–7004. DOIPubMedGoogle Scholar

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1These authors contributed equally to this article.

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