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Volume 12, Number 4—April 2006
Dispatch

MassTag Polymerase Chain Reaction for Differential Diagnosis of Viral Hemorrhagic Fevers

Gustavo Palacios*1, Thomas Briese*1Comments to Author , Vishal Kapoor*, Omar Jabado*, Zhiqiang Liu*, Marietjie Venter†, Junhui Zhai*, Neil Renwick*, Allen Grolla‡, Thomas W. Geisbert§, Christian Drosten¶, Jonathan S. Towner#, Jingyue Ju*, Janusz Paweska**, Stuart T. Nichol#, Robert Swanepoel**, Heinz Feldmann‡††, Peter B. Jahrling‡‡, and W. Ian Lipkin*
Author affiliations: *Columbia University, New York, New York, USA; †University of Pretoria and National Health Laboratory Services, Pretoria, South Africa; ‡Public Health Agency of Canada, Winnipeg, Manitoba, Canada; §United States Army Medical Research Institute of Infectious Diseases, Fort Detrick, Frederick, Maryland, USA; ¶Bernhard-Nocht-Institute of Tropical Medicine, Hamburg, Germany; #Centers for Disease Control and Prevention, Atlanta, Georgia, USA; **National Institute for Communicable Diseases, Sandringham, South Africa; ††University of Manitoba, Winnipeg, Manitoba, Canada; ‡‡National Institutes of Allergy and Infectious Diseases Integrated Research Facility, Fort Detrick, Frederick, Maryland, USA

Main Article

Table 2

Sensitivity of detection with synthetic RNA standards

Pathogen* Detection threshold (RNA copies)†
ZEBOV 20
SEBOV 20
MARV 20
LASV 20
RVFV 20
CCHFV 50
HNTV 20
SEOV 50
YFV 20
KFDV 20

*ZEBOV, Ebola Zaire virus; SEBOV, Ebola Sudan virus; MARV, Marburg virus; LASV, Lassa virus; RVFV, Rift Valley fever virus; CCHV, Crimean-Congo hemorrhagic fever virus; HNTV, Hantaan virus; SEOV, Seoul virus; YFV, yellow fever virus; KFDV, Kyasanur Forest disease virus.
†RNA copies refers to the number of molecules subjected to reverse transcription; half of the reverse transcription reaction was then used for polymerase chain reaction amplification.

Main Article

1These authors contributed equally to this article.

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