Volume 12, Number 7—July 2006
Dispatch
Replicon Typing of Plasmids Encoding Resistance to Newer β-Lactams
Alessandra Carattoli*
, Vivi Miriagou†, Alessia Bertini*, Alexandra Loli†, Celine Colinon‡, Laura Villa*, Jean M. Whichard§, and Gian Maria Rossolini‡
, Vivi Miriagou†, Alessia Bertini*, Alexandra Loli†, Celine Colinon‡, Laura Villa*, Jean M. Whichard§, and Gian Maria Rossolini‡
Figure
Figure. Restriction enzyme analysis of plasmids analyzed in this study. Numbers and letters above each lane indicate strain reference names as defined in the Table. M is the1-kb Plus DNA ladder (Gibco-BRL, Gaithersburg, MD, USA). Plasmids were transferred by conjugation or transformation in Escherichia coli K12 strains and purified by Midi-prep purification kit. Plasmids were digested with restriction endonuclease PstI and separated by agarose (1%) gel electrophoresis in 1× TBE buffer. DNA was stained with ethidium bromide and visualized under UV light. The replicons and the β-lactamase genes carried by each group of plasmids are indicated below the figure.
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