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Volume 12, Number 7—July 2006
Research

Tickborne Arbovirus Surveillance in Market Livestock, Nairobi, Kenya

Rosemary Sang*, Clayton Onyango*, John Gachoya*, Ernest Mabinda*, Samson Konongoi*, Victor Ofula*, Lee Dunster*, Fred Okoth*, Rodney Coldren†, Robert B. Tesh‡, Amelia Travassos da Rosa‡, Stacy Finkbeiner§, David Wang§, Mary Crabtree¶Comments to Author , and Barry Miller¶
Author affiliations: *Kenya Medical Research Institute, Nairobi, Kenya; †United States Army Medical Research Unit, Nairobi, Kenya; ‡University of Texas Medical Branch, Galveston, Texas, USA; §Washington University School of Medicine, Saint Louis, Missouri, USA; ¶Centers for Disease Control and Prevention, Fort Collins, Colorado, USA

Main Article

Table 1

Reverse transcription–polymerase chain reaction primers used for arbovirus isolation, Nairobi, Kenya

Virus target Target gene Primer designation Primer sequences*
Crimean-Congo hemorrhagic fever N CCHF F2 TGGACACCTTCACAAACTC
CCHF R3 GACAAATTCCCTGCACCA
Dugbe N DG S1 TCTCAAAGACAAACGTGCCGCAG
DG S5 TGCAACAACTGGATGTGTGA
Nairobi sheep disease N NSD 12f GAATGGTCGAACGTGGAC
NSD 16r TGCTGTCAGGACACCAGG
Thogoto N THO NF CCTGCAGGGGCGGAAGTTATG
THO NR AAAATCCTCGCAGTTGGCTATCA
Flaviviruses NS5 FLAVI fu2 GCTGATGACACCGCCGGCTGGGACAC
FLAVI cfd3 AGCATGTCTTCCGTGGTCATCCA
Bunyamwera and California serogroups N BCS82C ATGACTGAGTTGGAGTTTCATGATGTCGC
BCS332V TGTTCCTGTTGCCAGGAAAAT
Rift Valley fever G2 RVF-M-727f GGAACCCCTGCATGAAAGAGA
RVF-M-1565r CGATCCTGTGACGCAAACTC
Dhori N DHO NF2 TGGTACCCTTTTCTTGCTTCACTCC
DHO NR2 TGCTCTTCCTCGGCTCAAACACCA
Babanki C/E3 BAB 1007f TGGCCATGGAAGGTAAGGTAAT
BAB 1569r TATGGCGTTGAGCAGGGTATC
Hazara N HAZ 803f CTGGTTGAGCTAGAGGGGAAAGACG
HAZ 1304r GGGCGGCATCATCGGGACTG
Coxsackie B4 Pol COX 6749f ACTTTGTGAGAGGGGGTATGC
COX 7151r ACGTGGTATTGGGTGTTTTT
Koutango NS5 KOU 176f TCAGGGAGGTGGGAGGTAAAC
KOU 734r TCATGCCATCCAACAGAAGGT
Saboya NS5 SAB 226f GCAGGCTGGGACACAAAGAT
SAB 815r CTACAAGGGGCAATGATGGTTC
Ndelle l3 NDE 655f GGGGTTTTCTGGCTAATGTCAC
NDE 1238r GGGCCTGTCCAGTCTTTTTG
Middleburg E2 MID 1939f TACATGCCCCGAAGGTGACT
MID 2458r CGGGATGGTGTTCGGTAAAG

*Primer sequences 5´–3´.

Main Article

Page created: December 19, 2011
Page updated: December 19, 2011
Page reviewed: December 19, 2011
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