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Volume 12, Number 9—September 2006
Research

Predominance of Ancestral Lineages of Mycobacterium tuberculosis in India

M. Cristina Gutierrez*1, Niyaz Ahmed†1, Eve Willery‡, Sujatha Narayanan§, Seyed E. Hasnain†, Devendra S. Chauhan¶, Vishwa M. Katoch¶, Véronique Vincent*, Camille Locht‡, and Philip Supply‡Comments to Author 
Author affiliations: *Institut Pasteur, Paris, France; †Center for DNA Fingerprinting and Diagnostics, Hyderabad, India; ‡Institut Pasteur de Lille, Lille, France; §Tuberculosis Research Center, Chennai, India; ¶National Jalma Institute for Leprosy and Other Mycobacterial Diseases, Agra, India

Main Article

Table 3

Conditions for multiplex PCRs of 9 VNTR loci*

Multiplex Locus Conventional
designation† VNTR length (bp) MgCl2 (mmol) PCR primer pairs
(5´ to 3´, with labeling indicated)
Mix E 46 VNTR 2347 57 1.5 GCCAGCCGCCGTGCATAAACCT (Fam)
AGCCACCCGGTGTGCCTTGTATGAC
48 VNTR 2461 57 ATGGCCACCCGATACCGCTTCAGT (Vic)
CGACGGGCCATCTTGGATCAGCTAC
49 VNTR 3171 54 GGTGCGCACCTGCTCCAGATAA (Ned)
GGCTCTCATTGCTGGAGGGTTGTAC
Mix F 42 VNTR 0424 51 1.5 CTTGGCCGGCATCAAGCGCATTATT
GGCAGCAGAGCCCGGGATTCTTC (Fam)
43 VNTR 0577 58 CGAGAGTGGCAGTGGCGGTTATCT (Vic)
AATGACTTGAACGCGCAAATTGTGA
44 VNTR 1895 57 GTGAGCAGGCCCAGCAGACT (Ned)
CCACGAAATGTTCAAACACCTCAAT
Mix G 47 VNTR 2401 58 3.0 CTTGAAGCCCCGGTCTCATCTGT (Fam)
ACTTGAACCCCCACGCCCATTAGTA
52 VNTR 3690 58 CGGTGGAGGCGATGAACGTCTTC (Vic)
TAGAGCGGCACGGGGGAAAGCTTAG
53 VNTR 4156 59 TGACCACGGATTGCTCTAGT
GCCGGCGTCCATGTT (Ned)

*VNTR, variable-number tandem repeats.
†VNTR 0577, 2461, 4156, 1895 correspond to ETRC, ETRB, QUB 4156 and 1895, respectively (23,24). The conditions for PCR amplification were the same as in (30).

Main Article

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1These authors contributed equally to this article.

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