Volume 13, Number 1—January 2007
Research
Tickborne Relapsing Fever Diagnosis Obscured by Malaria, Togo
Table 1
Primers used for cloning, PCR, and DNA sequencing
Primer | Sequence (5′→3′)* | Reference |
---|---|---|
Br_GlpQ_3_BamHI | GGCGGATCCGCTTGACCAGTTGCTCCTCCGC | (16) |
Br_GlpQ_5_XhoI | GCCGCTCGAGGAAAAGAAAATGCAAAAATAAATAAA | (16) |
GlpQ For | GGTATGCTTATTGGTCTTC | Present study |
GlpQ Rev | TTGTATCCTCTTGTAATTG | Present study |
GlpQ1 | CAAATCACTAAGCCTTAGCGAAAGAT | Present study |
GlpQ2 | ATCTGTTGGTGCTTCTTCCCAGT | Present study |
GlpQ3 | CAGGGAAAATTGATAATGCTTGTTGG | Present study |
GlpQ4 | CTGCTAATGTGAAATCGACGGAATA | Present study |
Nested_1_F | AGAGTTTGATCCTGGCTTAG | (15) |
Nested_1_R | CTTGCATATCCGCCTACTCA | (15) |
Nested_2_F | GGCTTAGAACTAACGCTGGCA | (15) |
Nested_2_R | CTGCTGGCACGTAATTAGCC | (15) |
*Sequences in boldface indicate restriction sites for BamH1 and XhoI.
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