Volume 13, Number 10—October 2007
Dispatch
Rapid Field Immunoassay for Detecting Antibody to Sin Nombre Virus in Deer Mice
, Charles H. Calisher†, Tiffany R. Richens†, Audrey A. Rich*, Jeffrey B. Doty†, Mark T. Hughes†, and Barry J. Beaty†
Table
Comparison of results of PAGEIA and standard EIA for detection of antibody to Sin Nombre virus (SNV) in blood samples from 40 deer mice captured in southwest Colorado, 2006*
| Accession no. | Field PAGEIA score† | Laboratory PAGEIA OD‡ | Laboratory PAGEIA score§ | Standard EIA OD¶ | Standard EIA score# |
|---|---|---|---|---|---|
| HA-2548 | 3+ | 1.254 | Pos | 0.903/0.066 | Pos |
| HA-2552 | 4+ | 2.406 | Pos | 1.083/0.113 | Pos |
| HA-2554 | 4+ | 1.788 | Pos | 1.395/0.058 | Pos |
| HA-2558 | 4+ | 2.383 | Pos | 1.462/0.055 | Pos |
| HA-2560 | 4+ | 1.913 | Pos | 1.378/0.086 | Pos |
| HA-2564 | 0 | 0.001 | Neg | 0.327/0.055 | Pos |
| HA-2565 | 1+ | 0.236 | Pos | 0.715/0.046 | Pos |
| HA-2567 | 4+ | 2.123 | Pos | 1.485/0.080 | Pos |
| HB-2604 | 4+ | 2.065 | Pos | 1.161/0.067 | Pos |
| HB-2608 | 2+ | 0.855 | Pos | 0.653/0.095 | Pos |
| HB-2612 | 1+ | 0.282 | Pos | 1.136/0.071 | Pos |
| HB-2616 | 1+ | 0.311 | Pos | 0.458/0.001 | Pos |
| HB-2617 | 2+ | 0.517 | Pos | 0.819/0.008 | Pos |
| HB-2618 | 2+ | 0.494 | Pos | 1.085/0.009 | Pos |
| HB-2622 | 3+ | 1.254 | Pos | 1.519/0.029 | Pos |
| HB-2628 | 1+ | 0.493 | Pos | 0.220/0.082 | Neg |
| HB-2630 | 3+ | 1.609 | Pos | 0.681/0.008 | Pos |
| HA-2570 | 4+ | 1.970 | Pos | 0.389/0.024 | Pos |
| HA-2578 | 4+ | 2.101 | Pos | 1.185/0.017 | Pos |
| HB-2642 | 4+ | 2.784 | Pos | 1.294/0.063 | Pos |
| HA-2601 | 4+ | 2.699 | Pos | 0.921/0.121 | Pos |
| HA-2609 | 2+ | 0.608 | Pos | 0.228/0.042 | Neg |
| HA-2612 | 4+ | 2.482 | Pos | 1.072/0.085 | Pos |
| HA-2616 | 1+ | 0.331 | Pos | 0.076/0.059 | Neg |
| HB-2681 | 3+ | 0.977 | Pos | 1.392/0.048 | Pos |
| HB-2682 | 3+ | 1.095 | Pos | 1.326/0.042 | Pos |
| HA-2634 | 4+ | 3.010 | Pos | 0.863/0.014 | Pos |
| HA-2647 | 4+ | 2.824 | Pos | 0.720/0.023 | Pos |
| HA-2666 | 4+ | 2.682 | Pos | 0.477/0.028 | Pos |
| HB-2706 | 1+ | 0.836 | Pos | 0.324/0.032 | Pos |
| HB-2710 | 2+ | 0.664 | Pos | 0.155/0.035 | Neg |
| HB-2712 | 3+ | 1.599 | Pos | 0.345/0.033 | Pos |
| HB-2717 | 1+ | 1.098 | Pos | 0.293/0.027 | Pos |
| HB-2720 | 3+ | 2.581 | Pos | 0.630/0.039 | Pos |
| TS-0830–6 | 2+ | 0.889 | Pos | 0.799/0.097 | Pos |
| TS0830–7 | 1+ | 0.000 | Neg | 0.030/0.033 | Neg |
| TS-0830–8 | 3+ | 2.014 | Pos | 0.800/0.024 | Pos |
| TS-0830–9 | 4+ | 1.949 | Pos | 0.884/0.054 | Pos |
| TS-0830–18 | 4+ | 2.112 | Pos | 1.180/0.021 | Pos |
| TS-0830–20 | 3+ | 1.427 | Pos | 0.820/0.072 | Pos |
*PAGEIA, protein-A/G horseradish peroxidase enzyme-linked immunosorbent assay; EIA, enzyme immunoassay; OD, optical density; Pos, positive; Neg, negative.
†Field scores were based upon visual inspection without instrumentation, with 0 as negative, 1+ as very weak, 2+ as weak, 3+ as strong, and 4+ as very strong, relative to positive and negative control samples.
‡Field-collected samples were retested by PAGEIA under laboratory conditions and the OD reported here. The instrument was blanked on the negative control sample.
§OD >0.200 above the negative control was considered positive.
¶For laboratory EIA, OD was recorded for diluted (1:100) blood with both SNV antigen (numerator) and control antigen (denominator).
#A sample tested with SNV antigen and having an OD >0.300 was considered positive if the OD of that sample with the control antigen was <0.150. In regard to sample HBV-2717, the OD of the laboratory EIA with antigen was 0.293, very near the acceptable minimum, and the background was 0.027, which is very low; this sample was considered provisionally positive.