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Volume 13, Number 12—December 2007
Dispatch

Enhanced Subtyping Scheme for Salmonella Enteritidis

Jie Zheng*, Christine E. Keys†, Shaohua Zhao‡, Jianghong Meng*, and Eric W. Brown†Comments to Author 
Author affiliations: *University of Maryland, College Park, Maryland, USA; †US Food and Drug Administration, College Park, Maryland, USA; ‡US Food and Drug Administration, Laurel, Maryland, USA;

Main Article

Table 1

Pulsed-field gel electrophoresis run conditions for 6 restriction enzymes used to subtype Salmonella Enteritidis*

Enzyme Digestion temperature, °C Enzyme units, per plug Run time, h† Initial switch time, s Final switch time, s
XbaI 37 50 19 2.16 63.8
BlnI 37 30 19 2.16 63.8
SpeI 37 30 20.5 5 25
SfiI 50 30 20.5 5 25
PacI 37 30 20.5 0.1 15
NotI 37 30 20.5 0.1 15

*All digestion times were 2–3 h.
†Digestions were separated in 1% agarose gels (Cambrex, Baltimore, MD, USA) at 6V/cm by using a CHEF-Mapper (Bio-Rad, Hercules, CA, USA).

Main Article

Page created: July 06, 2010
Page updated: July 06, 2010
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The conclusions, findings, and opinions expressed by authors contributing to this journal do not necessarily reflect the official position of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.
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