Volume 13, Number 12—December 2007
Dispatch
Viable Newcastle Disease Vaccine Strains in a Pharmaceutical Dump
Antonella Amendola*
, Silvia Bianchi*, Marta Canuti*, Alessandra Zappa*, Giovanna Zanoni†, Raffaella Koncan†, Elisabetta Tanzi*, Giuseppe Cornaglia†, Alessandro Remo Zanetti*, and Giuseppe Tridente†
, Silvia Bianchi*, Marta Canuti*, Alessandra Zappa*, Giovanna Zanoni†, Raffaella Koncan†, Elisabetta Tanzi*, Giuseppe Cornaglia†, Alessandro Remo Zanetti*, and Giuseppe Tridente†
Table
Comparison of Newcastle disease virus sequences
| Classification* | Amino acid sequence |
|---|---|
| Lentogenic | 112(G/E)(R/K)Q(G/E)RL117 |
| Velogenic | 112(R/K)RQ(R/K)RF117 |
| Strains† | Nucleotide/amino acid sequence |
| ISM-1, ISM-2 | GGG AGA CAG GGG CGC CTT 112G R Q G R L117 |
| ISM-3, ISM-4 | AGG AGA CAG AGA CGG TTT 112R R Q R R F117 |
*Amino acid sequences at the F protein cleavage site of lentogenic and velogenic strains of Newcastle disease virus.
†Nucleotide/amino acid sequences of the samples recovered from a dump at Istituto Sieroterapico Milanese (ISM), Milan, Italy, and analyzed in this study. ISM-1 and ISM-2 (GenBank accession nos. EU082818 and EU082819, respectively) were recovered in lyophilized form; ISM-3 and ISM-4 (GenBank accession nos. EU082820 and EU082821, respectively) were recovered in liquid form. The cleavage site sequences of ISM-1 and ISM-2 are typical of lentogenic strains; the cleavage site sequences of ISM-3 and ISM-4 are typical of velogenic strains.
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