Volume 13, Number 12—December 2007
Viable Newcastle Disease Vaccine Strains in a Pharmaceutical Dump
|Classification*||Amino acid sequence|
|Strains†||Nucleotide/amino acid sequence|
|ISM-1, ISM-2||GGG AGA CAG GGG CGC CTT 112G R Q G R L117|
|ISM-3, ISM-4||AGG AGA CAG AGA CGG TTT 112R R Q R R F117|
*Amino acid sequences at the F protein cleavage site of lentogenic and velogenic strains of Newcastle disease virus.
†Nucleotide/amino acid sequences of the samples recovered from a dump at Istituto Sieroterapico Milanese (ISM), Milan, Italy, and analyzed in this study. ISM-1 and ISM-2 (GenBank accession nos. EU082818 and EU082819, respectively) were recovered in lyophilized form; ISM-3 and ISM-4 (GenBank accession nos. EU082820 and EU082821, respectively) were recovered in liquid form. The cleavage site sequences of ISM-1 and ISM-2 are typical of lentogenic strains; the cleavage site sequences of ISM-3 and ISM-4 are typical of velogenic strains.