In Vitro Cell Culture Infectivity Assay for Human Noroviruses
Timothy M. Straub*

, Kerstin Höner zu Bentrup†, Patricia Orosz Coghlan‡, Alice Dohnalkova*, Brooke K. Mayer*
1, Rachel A. Bartholomew*, Catherine O. Valdez*, Cynthia J. Bruckner-Lea*, Charles P. Gerba‡, Morteza A. Abbaszadegan§, and Cheryl A. Nickerson†
1
Author affiliations: *Pacific Northwest National Laboratory, Richland, Washington, USA; †Tulane University School of Medicine, New Orleans, Louisiana, USA; ‡University of Arizona, Tucson, Arizona, USA; §Arizona State University, Tempe, Arizona, USA;
Main Article
Table 3
Relative increases in viral RNA as measured by limiting dilution PCR*
Effective dilution of working viral stock applied to cells | Hours postinfection
|
---|
1 | 24 | 66 | 72 |
---|
1:10
| +
| +
| +
| ND
|
1:100
| +
| +
| +
| ND
|
1:1,000
| -
| -
| +
| +
|
Negative control | - | - | - | - |
Main Article
Page created: June 29, 2010
Page updated: June 29, 2010
Page reviewed: June 29, 2010
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