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Volume 13, Number 3—March 2007
Dispatch

Mouse-to-Human Transmission of Variant Lymphocytic Choriomeningitis Virus

Sébastien Emonet*, Karine Retornaz†, Jean-Paul Gonzalez‡, Xavier de Lamballerie*§, and Rémi N. Charrel*‡Comments to Author 
Author affiliations: *Université de la Méditerranée, Marseille, France; †Assistance Publique–Hôpitaux de Marseille Nord, Marseille, France; ‡Institut de Recherche pour le Développement, Bangkok, Thailand; §Assistance Publique–Hôpitaux de Marseille Timone, Marseille, France;

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Table

Detection of LCMV in mice trapped at patient’s home and in patient CSF*

SampleSystem 1
System 2System 3Virus isolationInterpreted result†
First-round PCRSecond-round PCR
Human
CSF 1–++NTNT‡+
CSF 2–++NTNT+
Mouse
1–+++–+
2––++++
3––––––
4–++–++
5–++–++
6–++–++
7–+++–+
8++++–+
9++++++
10––++++
11–+++–+
12§–+++++
13–+++++
14––––––
15––++++
16–+++++
17–+++–+
18++++++
19––+–++
20–+++++

*LCMV, lymphocytic choriomeningitis virus; CSF, cerebrospinal fluid, NT, not tested.
†An animal was considered infected with LCMV if virus was isolated either in Vero cells or results of at least 2 of 3 PCRs were positive.
‡After diagnostic tests were performed from CSF, no material was available to attempt virus isolation.
§Virus strain characterized by full-length genome sequencing (GenBank accession nos. DQ286931 and DQ286932 for S and L RNA, respectively).

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Page created: July 08, 2010
Page updated: July 08, 2010
Page reviewed: July 08, 2010
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